Possible misidentification of species in the Pseudomonas fluorescens lineage as Burkholderia pseudomallei and Francisella tularensis, and emended descriptions of Pseudomonas brenneri,Pseudomonas gessardii and Pseudomonas proteolytica
| Autor: | Daan W. Notermans, Frans A. G. Reubsaet, Erik Reinders, Maaike J. C. van den Beld |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Burkholderia pseudomallei 030106 microbiology Pseudomonas brenneri Pseudomonas fluorescens Microbiology 03 medical and health sciences Species Specificity Water Supply Pseudomonas RNA Ribosomal 16S Francisella tularensis Phylogeny Ecology Evolution Behavior and Systematics Netherlands biology Fatty Acids Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Pseudomonas proteolytica Pseudomonas gessardii Water Microbiology Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:3420-3425 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | Bacteria were isolated from an industrial water circuit in the Netherlands. These strains were identified using API 20 NE as possible, or likely, Burkholderia pseudomallei. With VITEK 2 some of these strains scored 'low discrimination' for Francisella tularensis, amongst others. A total of twenty-six strains were assigned to the species Pseudomonas brenneri, Pseudomonas gessardii or Pseudomonas proteolytica. Because of the possibility of misidentification of these environmental species as medical- and public-health relevant B. pseudomallei and F. tularensis, an emended description, based on tests results more customarily used in clinical laboratories, was suitable. For this reason, the strains in this study, including the type strains DSM 15294T, DSM 17152T and DSM 15321T, were subjected to a polyphasic identification procedure. This procedure consisted of multiple phenotypic tests, fatty acid analysis, 16S rDNA sequence analysis, matrix-assisted laser desorption ionization time-of-flight mass spectronomy and various species-specific molecular tests. Based on the results of the polyphasic procedures, the species descriptions of P. brenneri, P. gessardii and P. proteolytica have been emended. |
| Databáze: | OpenAIRE |
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