Oxidation of macrophage membrane cholesterol by intracellular Rhodococcus equi
| Autor: | Alan W. Bernheimer, Regina Linder |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Erythrocytes
Cholesterol oxidase Corynebacterium pseudotuberculosis Biology Microbiology Cell Line Membrane Lipids Mice chemistry.chemical_compound Species Specificity Rhodococcus equi medicine Animals Virulence General Veterinary Cholesterol Oxidase Cholesterol Phospholipase D Macrophages Cell Membrane General Medicine medicine.disease biology.organism_classification Hemolysis Biochemistry chemistry Cytokines bacteria Oxidation-Reduction Bacteria Intracellular |
| Zdroj: | Veterinary Microbiology. 56:269-276 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(97)00095-3 |
| Popis: | Phagocytic uptake by cultured mouse macrophages (PD388D1) of a virulent strain (ATCC 33701) of Rhodococcus equi producing substantial cholesterol oxidase was accompanied by intracellular survival of the bacteria, and enzymatic oxidation of macrophage membrane cholesterol. A non-virulent strain (4219) lacking cholesterol oxidase was largely eliminated from the macrophages and did not bring about oxidation of membrane cholesterol. When R. equi 33701 was co-phagocytosed with Corynebacterium pseudotuberculosis there was a significant enhancement (10-fold) in the amount of oxidation product (4-cholesten-3-one) generated. R. equi and C. pseudotuberculosis are cooperative partners in the hemolysis of sheep erythrocytes, traceable to the cholesterol oxidase of the former, and phospholipase D of the latter. Results are discussed relative to the role of cooperative cytotoxins in damage to host tissue by bacterial pathogens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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