Ferroelectric surfaces for cell release
| Autor: | Gunnar Kratz, Josefin Nissa, Daniel Simon, Susanna Lönnqvist, Anurak Sawatdee, Henrik Toss, Magnus Berggren, Simone Fabiano, David Nilsson |
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| Rok vydání: | 2017 |
| Předmět: |
Cell
Nanotechnology 02 engineering and technology Common method Electrical Engineering Electronic Engineering Information Engineering 010402 general chemistry Klinisk vetenskap 01 natural sciences Materials Chemistry medicine Fysik Elektroteknik och elektronik Chemistry Mechanical Engineering Metals and Alloys Clinical Science 021001 nanoscience & nanotechnology Condensed Matter Physics Ferroelectricity In vitro 0104 chemical sciences Electronic Optical and Magnetic Materials medicine.anatomical_structure Mechanics of Materials Physical Sciences Biophysics 0210 nano-technology |
| Zdroj: | Synthetic Metals. 228:99-104 |
| ISSN: | 0379-6779 |
| Popis: | Adherent cells cultured in vitro must usually, at some point, be detached from the culture substrate. Presently, the most common method of achieving detachment is through enzymatic treatment which breaks the adhesion points of the cells to the surface. This comes with the drawback of deteriorating the function and viability of the cells. Other methods that have previously been proposed include detachment of the cell substrate itself, which risks contaminating the cell sample, and changing the surface energy of the substrate through thermal changes, which yields low spatial resolution and risks damaging the cells if they are sensitive to temperature changes. Here cell culture substrates, based on thin films of the ferroelectric polyvinylidene fluoride trifluoroethylene (PVDF-TrFE) co-polymer, are developed for electroactive control of cell adhesion and enzyme-free detachment of cells. Fibroblasts cultured on the substrates are detached through changing the direction of polarization of the ferroelectric substrate. The method does not affect subsequent adhesion and viability of reseeded cells. Funding agencies: Swedish Governmental Agency for Innovation Systems (VINNOVA) [2010-00507]; Knut and Alice Wallenberg Foundation; Onnesjo Foundation |
| Databáze: | OpenAIRE |
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