Identification of Susceptibility Genes in Castanea sativa and Their Transcription Dynamics following Pathogen Infection
| Autor: | Emile Cavalet-Giorsa, Paolo Gonthier, Roberto Botta, Daniela Torello Marinoni, Andrea Moglia, Vera Pavese |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine chestnut Plant Science Phytophthora cinnamomi 01 natural sciences Microbiology 03 medical and health sciences Chestnut blight susceptibility genes Cryphonectria Cryphonectria parasitica Gene Pathogen Ecology Evolution Behavior and Systematics Ecology biology Phylogenetic tree Inoculation fungi Botany food and beverages Chestnut Susceptibility genes biology.organism_classification 030104 developmental biology QK1-989 Phytophthora 010606 plant biology & botany |
| Zdroj: | Plants, Vol 10, Iss 913, p 913 (2021) Plants Volume 10 Issue 5 |
| ISSN: | 2223-7747 |
| Popis: | Castanea sativa is one of the main multipurpose tree species valued for its timber and nuts. This species is susceptible to two major diseases, ink disease and chestnut blight, caused by Phytophthora spp. and Cryphonectria parasitica, respectively. The loss-of-function mutations of genes required for the onset of pathogenesis, referred to as plant susceptibility (S) genes, are one mechanism of plant resistance against pathogens. On the basis of sequence homology, functional domain identification, and phylogenetic analyses, we report for the first time on the identification of S-genes (mlo1, dmr6, dnd1, and pmr4) in the Castanea genus. The expression dynamics of S-genes were assessed in C. sativa and C. crenata plants inoculated with P. cinnamomi and C. parasitica. Our results highlighted the upregulation of pmr4 and dmr6 in response to pathogen infection. Pmr4 was strongly expressed at early infection phases of both pathogens in C. sativa, whereas in C. crenata, no significant upregulation was observed. The infection of P. cinnamomi led to a higher increase in the transcript level of dmr6 in C. sativa compared to C. crenata-infected samples. For a better understanding of plant responses, the transcript levels of defense genes gluB and chi3 were also analyzed. |
| Databáze: | OpenAIRE |
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