Evaluation of two immunoassay procedures for drug testing in hair samples
| Autor: | Katrin M. Kirschbaum, Frank Musshoff, Burkhard Madea, Hans Sachs, C. Herzfeld, K. Graumann |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Narcotics
medicine.diagnostic_test business.industry Hair analysis Poison control Enzyme-Linked Immunosorbent Assay Methamphetamine Pharmacology Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Pathology and Forensic Medicine Immunoenzyme Techniques Substance Abuse Detection Drug detection Forensic Toxicology Immunoassay medicine Humans Tetrahydrocannabinol business Amphetamine Law Hair Methadone medicine.drug |
| Zdroj: | Forensic Science International. 215:60-63 |
| ISSN: | 0379-0738 |
| DOI: | 10.1016/j.forsciint.2011.03.030 |
| Popis: | A preliminary initial enzyme-linked immunosorbent assay (LUCIO ® -Direct ELISA kit) and a preliminary DRI ® enzyme immunoassay were evaluated for drug detection in head hair with respect to lowered cutoff values recommended in Germany for the control of abstinence in cases of re-granting of drivers' licences. Following drug classes were included: cannabinoids, opiates, cocaine like substances, amphetamine, methamphetamine (and methylenedioxyamphetamines), methadone, and benzodiazepines. 759 analyses were performed using LUCIO ® -Direct ELISA kits and 936 analyses using DRI ® enzyme immunoassay tests. Sample size for each drug group and immunoassay test reached from 74 to 178. The LUCIO ® -Direct ELISA kit revealed a sensitivity of 91% for amphetamine up to 98% for methadone (methamphetamine 92%, cocaine 94%, opiates 94%, benzodiazepines 96%) and values of specificity of 72% for methadone up to 89% for amphetamine and benzodiazepines. The test was not useful for a preliminary screening for tetrahydrocannabinol (sensitivity of 65%) in consideration of a suggested cutoff of 0.02ng/mg. The DRI ® enzyme immunoassay test was only useful for morphine and cocaine testing at low recommended new cutoff values (0.1ng/mg) revealing sensitivities of 94% and 99%, respectively. |
| Databáze: | OpenAIRE |
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