Transcriptome expression profiles in prenatal pigs in relation to myogenesis
| Autor: | Vincenzo Russo, Marinus F.W. te Pas, J. Priem, M.H. Pool, M. Cagnazzo, Agnes de Wit, Roberta Davoli |
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| Přispěvatelé: | M.F.W. Te Pa, A.A.W. De Wit, J. Priem, M.Cagnazzo, R.Davoli, V.Russo, M.H.Pool. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Muscle tissue
Transcriptional Activation Myoblast proliferation medicine.medical_specialty Transcription Genetic Physiology Swine growth Down-Regulation Muscle Proteins selection Biology MyoD Muscle Development Biochemistry Polymerase Chain Reaction myod Andrology MYOGENESIS Internal medicine Gene expression medicine Myocyte Animals RNA Messenger Muscle Skeletal CIDC - Divisie Bacteriologie en TSE's Oligonucleotide Array Sequence Analysis Regulation of gene expression PIG Myogenesis Reproducibility of Results Cell Differentiation Cell Biology differentiation Microarray Analysis gene-expression TRANSCRIPTOME PROFILES Up-Regulation Endocrinology medicine.anatomical_structure Gene Expression Regulation Myogenic Regulatory Factors c2c12 C2C12 ID - Dier en Omgeving |
| Zdroj: | Journal of Muscle Research and Cell Motility 26 (2005) 2-3 Journal of Muscle Research and Cell Motility, 26(2-3), 157-165 |
| ISSN: | 0142-4319 |
| Popis: | Myogenesis, the formation of muscle fibers, is a complex process. Pigs have been selected for efficient muscle growth for the past decades making them interesting to study myogenesis. We studied expression profiles of genes known to affect myogenesis, muscle structural proteins, and energy metabolism in prenatal pigs from 14 to 91 days of gestation. Primary and secondary muscle fiber formation takes place during days 30-60 and 54-90 of gestation, respectively. Differential expression and expression levels of the genes were studied using microarray technology. Gene activation and repression profiles were studied counting the number of spots with detectable signal. The number of spots for muscle tissue structural protein genes showing upregulated expression increased constantly from day 14 until day 91 of gestation indicating continued activation of genes during this period. The mRNA expression level of the genes showed a peak around day 35 of gestation. The expression levels of genes affecting myogenic differentiation (stimulating and inhibiting) showed a peak at day 35 of gestation. The number of spots for differentiation-stimulating genes showing differential expression reaches a first peak around day 35 of gestation and a nadir at day 49 of gestation while the number of spots for differentiation-inhibiting genes reaches a nadir at day 35 of gestation. Myogenic differentiation seems less a matter of the expression level of genes affecting differentiation, but depends on the balance between the number of significantly activated genes for stimulating and inhibiting differentiation. Genes stimulating myoblast proliferation showed a small peak expression prior to day 35 of gestation indicating myoblast proliferation before differentiation. The number of spots and the expression levels of genes for glycolysis and ATP-metabolism are at a nadir around days 35 and 49-63 of gestation suggesting that the energy metabolism is low during fusion of myoblasts into multinucleated muscle fibers. |
| Databáze: | OpenAIRE |
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