Is there a relationship between PCNA expression and diabetic placental development during pregnancy?
| Autor: | Emin Turkay Korgun, Nuray Acar, Ismail Ustunel, Zeliha Sahin, Ramazan Demir, Sevil Cayli |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Histology Placenta Streptozocin Diabetes Mellitus Experimental Diabetes Complications Immunolabeling Western blot Pregnancy Proliferating Cell Nuclear Antigen Diabetes mellitus Internal medicine medicine Animals Rats Wistar biology medicine.diagnostic_test Cell Cycle Cell Biology General Medicine medicine.disease Immunohistochemistry Placentation Rats Proliferating cell nuclear antigen Endocrinology medicine.anatomical_structure biology.protein Pregnancy Animal Female |
| Zdroj: | Acta Histochemica. 110:408-417 |
| ISSN: | 0065-1281 |
| Popis: | We aimed to investigate the distribution pattern of proliferating cell nuclear antigen (PCNA) by immunohistochemistry and Western blot in placentas of control and diabetic rats at different stages of pregnancy. It is still not clear how proliferation is coordinated and how this coordination is affected by diabetes in the placenta. Diabetes was induced by streptozocin on the first day of pregnancy. Animals were sacrificed on days 11, 13, 17 and 21 of pregnancy. In control placentas immunolabeling intensity of PCNA was the highest on days 11 and 13 of pregnancy and decreased with progression of pregnancy. In the diabetic groups immunolabeling was less intense on days 11 and 13 of pregnancy compared to controls. However, in parallel with placental weights, PCNA immunopositivity was more intense in diabetic groups than control groups on days 17 and 21 of pregnancy, and the difference was statistically significant on day 17. According to Western blot data, on days 11 and 13 of pregnancy the amount of PCNA was greater in control groups than in the diabetics, whereas it was greater in diabetic groups than the controls on days 17 and 21 of pregnancy. We conclude that PCNA may play a role in abnormal placenta formation resulting from diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect