Effects of luteolin on spatial memory, cell proliferation, and neuroblast differentiation in the hippocampal dentate gyrus in a scopolamine-induced amnesia model
| Autor: | Jong Hwi Kim, Sung Min Nam, In Koo Hwang, Moo Ho Won, Woosuk Kim, Hyo Young Jung, Dae Young Yoo, Jung Hoon Choi, Yeo Sung Yoon |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Doublecortin Protein Aché Neurogenesis Scopolamine Hippocampal formation Pharmacology chemistry.chemical_compound Galantamine medicine Animals Luteolin Maze Learning Cell Proliferation Brain-derived neurotrophic factor Memory Disorders Brain-Derived Neurotrophic Factor Dentate gyrus Cell Differentiation General Medicine Acetylcholinesterase language.human_language Rats Neuroprotective Agents Neurology chemistry Anesthesia Dentate Gyrus language Amnesia Cholinesterase Inhibitors Lipid Peroxidation Neurology (clinical) Neuroblast differentiation medicine.drug |
| Zdroj: | Neurological Research. 35:813-820 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1179/1743132813y.0000000217 |
| Popis: | Luteolin, a common flavonoid from many plants, has various pharmacological activities, including a memory-improving effect. In this study, we investigated the effects of luteolin on spatial memory, cell proliferation, and neuroblast differentiation in the hippocampal dentate gyrus in a rat model of scopolamine (SCO)-induced amnesia.Scopolamine was subcutaneously administered for 28 days via an Alzet minipump (44 mg/ml delivered at 2·5 μl/h) along with a daily intraperitoneal administration of vehicle (saline) 10 mg/kg luteolin or 5 mg/kg galantamine (GAL) (a control drug for acetylcholinesterase (AChE) inhibitor) for 28 days.The administration of SCO significantly decreased the spatial alteration percentage in the Y-maze test compared to that in the vehicle (saline)-treated group. The administration of luteolin or GAL significantly improved the spatial alteration percentage compared to that in the SCO-treated group. Similarly, the administration of SCO significantly decreased the cell proliferation (Ki67-positive cells) and neuroblast differentiation (doubleocortin-positive cells) in the dentate gyrus. The administration of luteolin or GAL significantly mitigated the SCO-induced reduction of Ki67- and doublecortin-immunoreactive cells in the dentate gyrus. In addition, the administration of luteolin significantly decreased the lipid peroxidation (malondialdehyde (MDA) levels) and increased the brain-derived neurotrophic factor (BDNF) and AChE levels in the hippocampal homogenates compared to the SCO-treated group.These results suggest that the luteolin treatment improves the SCO-induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus. The mechanism underlying the amelioration of SCO-induced amnesia by luteolin may be associated with the increase in BDNF, acetylcholine, and the decrease in lipid peroxidation. |
| Databáze: | OpenAIRE |
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