Atherogenic and inflammatory profile of human arterial endothelial cells (HUAEC) in response to LDL subfractions
| Autor: | Cristina Bancells, Sonia Benitez, Jordi Ordóñez-Llanos, Manuel Reina, José Luis Sánchez-Quesada, Conxita de Castellarnau |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Endothelium Chemokine CXCL1 medicine.medical_treatment Clinical Biochemistry Protein Array Analysis Enzyme-Linked Immunosorbent Assay Inflammation Biochemistry Umbilical Arteries Umbilical vein Proinflammatory cytokine Internal medicine medicine Humans Interleukin 8 Interleukin 6 Cells Cultured Chemokine CCL2 biology Interleukin-6 Chemistry Growth factor Interleukin-8 Biochemistry (medical) Proto-Oncogene Proteins c-sis General Medicine Atherosclerosis Lipoproteins LDL medicine.anatomical_structure Endocrinology Culture Media Conditioned biology.protein lipids (amino acids peptides and proteins) Endothelium Vascular medicine.symptom Chemokines CXC Lipoprotein |
| Zdroj: | Clinica Chimica Acta. 376:233-236 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2006.07.024 |
| Popis: | Background Electronegative LDL (LDL(−)) is a minor modified LDL fraction present in plasma that has been demonstrated to be inflammatory in endothelial cells isolated from human umbilical vein (HUVEC). Methods A protein array able to measure 42 cytokines, chemokines and related compounds involved in atherogenesis was used to determine their release into the culture medium of human arterial endothelial cells (HUAEC) activated or not by two low-density lipoprotein (LDL) fractions isolated from human plasma by anion-exchange chromatography. Results The results of the protein array (confirmed using specific ELISAs for each induced factor) revealed that HUAEC in the absence of stimuli released small amounts of interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1) and growth-related oncogene (GRO). The major native LDL fraction (named LDL(+)) increased the release of these molecules and also those of interleukin 6 (IL-6) and GROα. Compared to LDL(+), the minor modified fraction, named electronegative LDL (LDL(−)), increased all these factors to a greater degree and also induced the release of granulocyte–monocyte colony-stimulating factor (GM-CSF) and platelet-derived growth factor B (PDGF-B). These results were confirmed by ELISA. Conclusions All these results indicate that, compared to LDL(+), LDL(−) fraction promotes not only the release of proinflammatory factors but also those of atherogenic factors in endothelial cells of arterial origin, thereby suggesting a new role for LDL(−) in atherogenesis. |
| Databáze: | OpenAIRE |
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