Extracellular wire tetrode recording in brain of freely walking insects
| Autor: | Peiyuan Guo, Alan J. Pollack, Joshua P. Martin, Adrienn G. Varga, Roy E. Ritzmann |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Brain activity and meditation Computer science General Chemical Engineering Video Recording Cockroaches Walking General Biochemistry Genetics and Molecular Biology Neural activity biology.animal Animals Movement (clockwork) Tetrode (biology) Neurons Cockroach General Immunology and Microbiology biology General Neuroscience Motor control Brain Electrodes Implanted Preferred walking speed Climbing Female Neuroscience Microelectrodes |
| Zdroj: | Journal of visualized experiments : JoVE. (86) |
| ISSN: | 1940-087X |
| Popis: | Increasing interest in the role of brain activity in insect motor control requires that we be able to monitor neural activity while insects perform natural behavior. We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allowed us to record activity from multiple neurons simultaneously while a tethered cockroach turned or altered walking speed. While a major advance, tethered preparations provide access to limited behaviors and often lack feedback processes that occur in freely moving animals. We now present a modified version of that technique that allows us to record from the central complex of freely moving cockroaches as they walk in an arena and deal with barriers by turning, climbing or tunneling. Coupled with high speed video and cluster cutting, we can now relate brain activity to various parameters of the movement of freely behaving insects. |
| Databáze: | OpenAIRE |
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