Differential innate immune responses induced by Mycoplasma hyopneumoniae and Mycoplasma hyorhinis in various types of antigen presenting cells
Autor: | Roman Othmar Braun, Bettina S. Trueeb, Peter Kuhnert, Artur Summerfield, Gaël Auray |
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Rok vydání: | 2019 |
Předmět: |
Mycoplasma hyorhinis
Swine Antigen-Presenting Cells medicine.disease_cause Microbiology 03 medical and health sciences Immune system Mycoplasma hyopneumoniae medicine Animals Mycoplasma Infections CD40 Antigens Antigen-presenting cell 030304 developmental biology Swine Diseases 0303 health sciences B-Lymphocytes CD40 Innate immune system 630 Agriculture General Veterinary biology 030306 microbiology Tumor Necrosis Factor-alpha Interleukin-2 Receptor alpha Subunit General Medicine Mycoplasma Dendritic Cells biology.organism_classification Immunity Innate Immunoglobulin M biology.protein Leukocytes Mononuclear 570 Life sciences Tumor necrosis factor alpha |
Zdroj: | Veterinary microbiology. 240 |
ISSN: | 1873-2542 |
Popis: | Mycoplasma (M.) hyopneumoniae is the etiological agent of enzootic pneumonia in pigs and is closely related to M. hyorhinis, which can be isolated from the healthy mucosal surfaces of the upper respiratory tract. In rare cases it can also cause arthritis and polyserositis. Since the innate immune system is an important first line of defense and promotes adaptive immune responses, we characterized the innate immune response of various antigen presenting cells (APCs) to M. hyopneumoniae and M. hyorhinis, which differ in their pathogenicity in vivo. Porcine peripheral blood mononuclear cells were infected with different multiplicities of infection (MOI) of live and inactivated porcine mycoplasmas. Both Mycoplasma species induced strong tumour necrosis factor (TNF) responses in monocytes, with a stronger activation by M. hyorhinis. This higher stimulatory activity was also confirmed for CD40 upregulation. Conventional and plasmacytoid dendritic cells (cDC and pDC, respectively) did not or poorly respond to mycoplasmas in terms of TNF expression but more efficiently in terms of CD40 upregulation. Again, these responses were generally stronger with M. hyorhinis than with M. hyopneumoniae. Both Mycoplasma species also activated B cells in terms of CD25 upregulation, proliferation, and IgM secretion. Interestingly, while the induction of CD25 and in particular proliferation was higher with M. hyorhinis, the IgM secretion did not differ between the two species with the exception of the highest dose of M. hyopneumoniae,which appeared to suppress IgM responses. Taken together, our results provide a comparative analysis of innate immune response with different porcine APCs and demonstrate Mycoplasma species-dependent differences, which could relate to their different pathogenicity in vivo. |
Databáze: | OpenAIRE |
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