Bacteria tolerant to colistin in coastal marine environment: Detection, microbiome diversity and antibiotic resistance genes' repertoire
| Autor: | Ana Maravić, Željana Fredotović, Hrvoje Kalinić, Mia Dželalija, Ivica Šamanić, Ana-Marija Bungur |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Colistin resistance
Coastal waters Extended-spectrum β-lactamase Marine environment Metagenome sequencing Mcr-1 gene Serratia Environmental Engineering Croatia Health Toxicology and Mutagenesis 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences 01 natural sciences beta-Lactamases RNA Ribosomal 16S Drug Resistance Bacterial medicine Environmental Chemistry Microbiome Gene 0105 earth and related environmental sciences Genetics biology Colistin Microbiota Public Health Environmental and Occupational Health Drug Resistance Microbial General Medicine General Chemistry biology.organism_classification 16S ribosomal RNA Pollution Anti-Bacterial Agents 020801 environmental engineering Multiple drug resistance Metagenomics Efflux Bacteria medicine.drug |
| DOI: | 10.1016/j.chemosphere.2021.130945 |
| Popis: | The global spread of mobilized colistin resistance (mcr) genes in clinical and natural environments dangerously diminishes the effectiveness of this last-resort antibiotic, becoming an urgent health threat. We used a multidisciplinary approach to detect mcr-1 gene and colistin (CL)-resistant bacteria in seawater from two Croatian public beaches. Illumina-based sequencing of metagenomic 16S rRNA was used to assess the taxonomic, functional, and antibiotic resistance genes (ARGs) profiling of the bacterial community tolerant to CL regarding different culture- based isolation methodologies. Data revealed that the choice of methodology alters the diversity and abundance of taxa accounting for the CL-resistance phenotype. The mcr-1 gene was identified by cloning and sequencing in one sample, representing the first report of mcr-1 gene in Croatia. Culturing of CL-resistant strains revealed their resistance phenotypes and concurrent production of clinically significant β-lactamases, such as CTX-M-15, CTX-M-3 and SHV-12. We also report the first identification of blaCTX-M-15 gene in Klebsiella huaxiensis and K. michiganensis, as well as the blaTEM- 1+CTX-M-3 in Serratia fonticola. ARGs profiles derived from metagenomic data and predicted by PICRUSt2, showed the highest abundance of genes encoding for multidrug efflux pumps, followed by the transporter genes accounting for the tetracycline, macrolide and phenicol resistance. Our study evidenced the multidrug resistance features of CL-tolerant bacterial communities thriving in surface beach waters. We also showed that combined application of the metagenomic approaches and culture-based techniques enabled successful detection of mcr- 1 gene, which could be underreported in natural environment. |
| Databáze: | OpenAIRE |
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