Multivalent aptamer-modified tetrahedral DNA nanocage demonstrates high selectivity and safety for anti-tumor therapy
| Autor: | Zhenghong Wu, Xiu Han, Xiaonan Ma, Li Shuyi, Yujie Jiang, Yu Zhang, Xiaole Qi, Ziheng Wu |
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| Rok vydání: | 2019 |
| Předmět: |
Aptamer
Mice Nude Antineoplastic Agents 02 engineering and technology Microscopy Atomic Force 010402 general chemistry 01 natural sciences Mice chemistry.chemical_compound Drug Delivery Systems Nanocages In vivo Cell Line Tumor Animals Humans DNA origami General Materials Science Molecular Targeted Therapy DNA Aptamers Nucleotide 021001 nanoscience & nanotechnology In vitro Nanostructures 0104 chemical sciences chemistry Doxorubicin MCF-7 Cells Nucleic acid Biophysics Nucleic Acid Conformation Female 0210 nano-technology Neoplasm Transplantation Intracellular |
| Zdroj: | Nanoscale. 11:339-347 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | The fabrication of aptamers on DNA nanostructures through the DNA origami method has shown great potential for in vitro and in vivo tumor targeting delivery. Herein, tetrahedral multivalent DNA (Td) nanocages modified with different numbers of MUC1-aptamers (nApt-Td) were obtained via the self-assembly of nucleic acid backbones containing the aptamer DNA fragment without complicated chemical modification steps. Then, doxorubicin (DOX) was encapsulated in the modified nApt-Td nanocages via non-covalent interactions. Due to the specific identification of the aptamers, the intracellular uptake studies demonstrated that the multivalent modified aptamers on the vertex of Td significantly enhanced the intracellular uptake efficiency in MCF-7 tumor cells, but reduce that of normal cells. The number of aptamers had a significant influence on the intracellular uptake efficiency of Td nApt-Td. Furthermore, improved in vivo systemic safety and decreased systemic toxicity accompanied with effective tumor inhibition were also demonstrated compared with the anti-tumor efficiency of free DOX. |
| Databáze: | OpenAIRE |
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