Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies
| Autor: | Anna Puścion-Jakubik, Patryk Nowakowski, Monika Grabia, Joanna Bielecka, Katarzyna Socha, Konrad Mielcarek, Jolanta Soroczyńska, Sylwia K. Naliwajko, Justyna Moskwa, Renata Markiewicz-Żukowska, Krystyna Gromkowska-Kępka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
photoaging Physiology medicine.medical_treatment Photoaging Clinical Biochemistry chemistry.chemical_element skin care Human skin RM1-950 nutricosmetics Biochemistry UV radiation fibroblasts medicine Food science Molecular Biology Chemistry food and beverages Biological activity Cell Biology medicine.disease young barley In vitro antioxidant properties Polyphenol Hordeum vulgare Therapeutics. Pharmacology Selenium |
| Zdroj: | Antioxidants, Vol 10, Iss 1402, p 1402 (2021) Antioxidants Volume 10 Issue 9 |
| ISSN: | 2076-3921 |
| Popis: | Young barley seems to be a promising material for use as nutricosmetic due to the presence of many biologically active compounds. The aim of this study was to evaluate the effect of Hordeum vulgare L. extracts on human skin fibroblasts exposed to ultraviolet radiation B (UVB) radiation. Analysis of the chemical composition showed a predominance of 9,12,15-octadecatrienoic acid. The quality assessment showed that young barley preparations have high total polyphenolic content (TPC) and favourable total antioxidant status (TAS). They also contain antioxidant elements such as zinc, copper, and selenium. Furthermore, the analyzed products were found to be safe in terms of toxic elements (lead, cadmium and mercury) and lack of cytotoxic effect of young barley extracts on cells. In vitro bioactivity assays showed that young barley extract increased the survival rate and accelerated the migration of fibroblasts in research models with UVB radiation. The application of both extracts caused an increase in DNA biosynthesis, and in the number of cells arrested in S phase. Moreover, an inhibitory effect of the tested extracts on the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) was observed. The results indicate that young barley extracts, due to protective as well as restorative effect, could potentially be used in the production of nutricosmetics and skin care products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|