UV-Visible spectroscopic effect on Haemoglobin & DNA degradation: A forensic approach
| Autor: | Suminder Kaur, Ritika Dalal, Vineeta Saini |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ultraviolet Rays
Sample (material) Blood Stains Forensic serology Biology Dna testing 01 natural sciences ABO Blood-Group System Specimen Handling Pathology and Forensic Medicine Hemoglobins 03 medical and health sciences 0302 clinical medicine DNA degradation Humans 030216 legal & forensic medicine Nucleic acid stability Chromatography Spectrum Analysis 010401 analytical chemistry DNA Degradation Necrotic DNA 0104 chemical sciences Indian subcontinent Forensic science Law |
| Zdroj: | Forensic Science International. 307:110078 |
| ISSN: | 0379-0738 |
| Popis: | In forensic casework, sample degradation is a largely an unsolved problem that exists due to poor preservation protocols. In Indian subcontinent, the forensic blood stains are commonly exposed to different ecological conditions for months before they are collected and transferred to the forensic laboratory for analysis. Under such conditions, the question of haemoglobin (Hb) & nucleic acid stability is of utmost importance. The biological evidence, especially the blood sample that underwent extreme conditions should further be retained in an appropriate storage facility until processed for the forensic serology/DNA testing. The present study provides the spectroscopic approach of analyzing Hb and DNA degradation of forensic blood samples preserved under varied conditions (tabulated and graphically). These samples were further subjected to and DNA analysis to assess the quality of sample based on different preservation conditions. At last, this study suggests the effective and appropriate preservation protocol of sample soon after its collection. |
| Databáze: | OpenAIRE |
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