Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers
| Autor: | Roi Gazit, Roshina Thapa, Leonid Olender |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
murine bone marrow
Myeloid QH301-705.5 CD34 Antigens CD34 Biology Transcriptome Mice Signaling Lymphocytic Activation Molecule Family Member 1 medicine Animals Biology (General) Progenitor cell cell sorting Myeloid Progenitor Cells Receptors IgG General Medicine myeloid progenitors Cell sorting Flow Cytometry Hematopoietic Stem Cells hematopoiesis Cell biology Haematopoiesis medicine.anatomical_structure Bone marrow Stem cell Cell Adhesion Molecules |
| Zdroj: | Cells, Vol 11, Iss 350, p 350 (2022) |
| ISSN: | 1556-5068 |
| DOI: | 10.2139/ssrn.3680823 |
| Popis: | Myeloid progenitors are intermediates between Hematopoietic Stem Cells (HSCs) and Myeloid effector progeny. In mouse bone marrow, they are part of the Lineage− cKit+ Sca1− (LK) compartment. To date, most researchers used CD34 and FcγR surface markers for the dissection of this compartment into various populations. Surprisingly, however, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we suggest using CD150 instead of FcγR. We re-analyzed published single-cell RNA-Seq data and found that CD34/CD150 provides better sub-populations separation, compared to the “classical” CD34/FcγR-based approach. We confirm our findings by independent FACS analysis. We demonstrate comparable differentiation potential of the newly-obtained LK sub-populations, like previous “classical” ones. Therefore, we suggest the CD34/CD150 gating strategy, utilizing commonly-used surface markers, as a robust and reproducible separation of the LK compartment into distinct sub-populations. |
| Databáze: | OpenAIRE |
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