Tobacco Hornworm (Manduca sexta) caterpillars as a novel host model for the study of fungal virulence and drug efficacy
| Autor: | Carolyn E. Williamson, Naomi Lyons, Amol C. Shetty, Isabel Softley, Andrew Balfour, Heath E. O’Brien, Stephanie Diezmann, Vincent M. Bruno |
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| Rok vydání: | 2020 |
| Předmět: |
Antifungal drug
Cryptococcus Infectious and parasitic diseases RC109-216 metschnikowia Saccharomyces manduca sexta saccharomyces Manduca fungal burden Candida albicans Candida 0303 health sciences Virulence biology antifungal drug Infectious Diseases Larva candida Metschnikowia host model Research Article Research Paper Microbiology (medical) animal structures food.ingredient Immunology Microbiology Manduca sexta 03 medical and health sciences food Animals 030304 developmental biology Cryptococcus neoformans 030306 microbiology Gene Expression Profiling fungi Fungi biology.organism_classification cryptococcus Disease Models Animal fungal virulence Mycoses caterpillar Parasitology |
| Zdroj: | Virulence, Vol 11, Iss 1, Pp 1075-1089 (2020) Lyons, N, Softley, I, Balfour, A, Williamson, C, O'Brien, H E, Shetty, A C, Bruno, V M & Diezmann, S 2020, ' Tobacco Hornworm (Manduca sexta) caterpillars as a novel host model for the study of fungal virulence and drug efficacy ', Virulence, vol. 11, no. 1, pp. 1075-1089 . https://doi.org/10.1080/21505594.2020.1806665 Virulence article-version (VoR) Version of Record |
| ISSN: | 2150-5608 2150-5594 |
| Popis: | The two leading yeast pathogens of humans, Candida albicans and Cryptococcus neoformans, cause systemic infections in >1.4 million patients worldwide with mortality rates approaching 75%. It is thus imperative to study fungal virulence mechanisms, efficacy of antifungal drugs, and host response pathways. While this is commonly done in mammalian models, which are afflicted by ethical and practical concerns, invertebrate models, such as wax moth larvae and nematodes have been introduced over the last two decades. To complement existing invertebrate host models, we developed fifth instar caterpillars of the Tobacco Hornworm moth Manduca sexta as a novel host model. These caterpillars can be maintained at 37°C, are suitable for injections with defined amounts of yeast cells, and are susceptible to the most threatening yeast pathogens, including C. albicans, C. neoformans, C. auris, and C. glabrata. Importantly, fungal burden can be assessed daily throughout the course of infection in a single caterpillar’s feces and hemolymph. Infected caterpillars can be rescued by treatment with antifungal drugs. Notably, these animals are large enough for weight to provide a reliable and reproducible measure of fungal disease and to facilitate host tissue-specific expression analyses. M. sexta caterpillars combine a suite of parameters that make them suitable for the study of fungal virulence. |
| Databáze: | OpenAIRE |
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