Messenger RNAs localized to distal projections of human stem cell derived neurons
| Autor: | Joyce W. Kamande, Raluca Dumitru, Rebecca L. Bigler, Anne Marion Taylor, Mark Niedringhaus |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Science Cellular differentiation ved/biology.organism_classification_rank.species Cell Culture Techniques Biology Bioinformatics RNA Transport Article Transcriptome 03 medical and health sciences Gene expression Humans RNA Messenger Model organism Cells Cultured Embryonic Stem Cells In Situ Hybridization Cerebral Cortex Neurons Messenger RNA Multidisciplinary ved/biology Stem Cells Computational Biology Cell Differentiation Translation (biology) Microfluidic Analytical Techniques Embryonic stem cell Axons Cell biology Gene Ontology 030104 developmental biology nervous system Medicine Axon guidance |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-00676-w |
| Popis: | The identification of mRNAs in distal projections of model organisms has led to the discovery of multiple proteins that are locally synthesized for functional roles such as axon guidance, injury signaling and regeneration. The extent to which local protein synthesis is conserved in human neurons is unknown. Here we used compartmentalized microfluidic chambers to characterize the transcriptome of distal projections of human embryonic stem cells differentiated using a protocol which enriched for glutamatergic neurons (hESC-neurons). Using gene expression analysis, we identified mRNAs proportionally enriched in these projections, representing a functionally unique local transcriptome as compared to the human neuronal transcriptome inclusive of somata. Further, we found that the most abundant mRNAs within these hESC-neuron projections were functionally similar to the axonal transcriptome of rat cortical neurons. We confirmed the presence of two well characterized axonal mRNAs in model organisms, β-actin and GAP43, within hESC-neuron projections using multiplexed single molecule RNA-FISH. Additionally, we report the novel finding that oxytocin mRNA localized to these human projections and confirmed its localization using RNA-FISH. This new evaluation of mRNA within human projections provides an important resource for studying local mRNA translation and has the potential to reveal both conserved and unique translation dependent mechanisms. |
| Databáze: | OpenAIRE |
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