Label-free and high-throughput biosensing of multiple tumor markers on a single light-addressable photoelectrochemical sensor
Autor: | Wangze Wu, Chengguo Hu, Juan Long, Juan Wang, Zhihong Liu |
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Rok vydání: | 2017 |
Předmět: |
Materials science
Fabrication CA-19-9 Antigen Light Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology Sulfides 010402 general chemistry 01 natural sciences Limit of Detection Neoplasms Biomarkers Tumor Electrochemistry Humans Electrodes Detection limit Energy conversion efficiency Substrate (chemistry) Electrochemical Techniques Equipment Design General Medicine 021001 nanoscience & nanotechnology Carcinoembryonic Antigen High-Throughput Screening Assays 0104 chemical sciences Colloidal gold Electrode alpha-Fetoproteins 0210 nano-technology Antibodies Immobilized Bismuth Biosensor Biotechnology Visible spectrum |
Zdroj: | Biosensors and Bioelectronics. 91:53-59 |
ISSN: | 0956-5663 |
Popis: | The sensitive and label-free detection of multiple biomarkers on a single electrode by photoelectrochemical (PEC) sensors based on light addressing strategies is very attractive for developing portable and high-throughput biosensing systems. The essential prerequisite of this proposal is the employment of uniform photovoltaic material modified electrodes with high conversion efficiency. Herein, a novel two-step constant potential deposition method for the rapid fabrication of bismuth sulfide film modified ITO electrodes (Bi2S3/ITO) was established. The produced Bi2S3/ITO, with excellent uniformity and high conversion efficiency in visible light ranges, was further modified with gold nanoparticles (AuNPs) and then divided into separated identical sensing zones by insulative paints. The adsorption-based immobilization of antibodies of three tumor markers, i.e., a-fetoprotein (AFP), carcinoembryonic antigen (CEA) and cancer antigen 19-9 (CA19-9), onto different sensing zones of the electrode and the further blocking with BSA established a label-free and light-addressable PEC sensor (LF-LAPECS), which can achieve the rapid and sensitive detection of these biomarkers with wide linear ranges, low detection limits and self-calibration ability. Moreover, the detection throughput can be conveniently improved by enlarging the size of the substrate electrode and increasing the number of separated sensing zones. The present work thus demonstrates the promising applications of PEC techniques for developing sensitive, time-saving, cost-effective and high-throughput biosensing methods. |
Databáze: | OpenAIRE |
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