| Autor: |
Chiche, Aurélie, Di-Cicco, Amandine, Sesma-Sanz, Laura, Bresson, Laura, Grange, Pierre De La, Glukhova, Marina, Faraldo, Marisa, Marie-Ange Deugnier |
| Rok vydání: |
2019 |
| DOI: |
10.6084/m9.figshare.7635059.v1 |
| Popis: |
Figure S2. Characteristics of the basal and luminal populations isolated from control and K5Cre;Trp53F/F adult virgin mice, and of mammospheres derived from control and p53-deficient luminal progenitors. (a) Expression levels of the luminal- and basal-specific keratins (Krt18 and Krt5) in control and p53-deficient Lu-pos cells, evaluated by qPCR. Data are the mean ± SEM of 4 separate preparations. (b) Hgf expression in control and p53-deficient Lu-neg, Lu-pos, and basal cell populations. The qPCR data are shown as mean ± SEM of 3 separate preparations. *p ≤ 0.05. (c) Size distribution of MS2 spheres generated by control and p53-deficient Lu-pos cells grown with or without HGF. Sphere areas were estimated in pixels on phase contrast pictures, using ImageJ software. Spheres of ≤ 400 and > 400 pixels were defined as small and large, respectively. Data are the mean ± SEM of 4 separate preparations. (d) Trp53 expression in MS1 and MS2 spheres derived from control and p53-deficient Lu-pos cells grown with or without HGF. The qPCR data are shown as mean ± SEM of 4 separate preparations. *p ≤ 0.05. (e) Expression levels of Trp53, Trp63, and Snai2 in basal cells isolated from control and K5Cre;Trp53F/F mutant adult virgin mice, evaluated by qPCR. Data are the mean ± SEM of 3 separate preparations. **p ≤ 0.01. (PDF 5273 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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