Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis

Autor: F.H.L. Kwaspen, H. Bulten, A. Broos, Frans C. S. Ramaekers, W.M.H. Debie, F.M.M. Smedts
Rok vydání: 1997
Předmět:
Pathology
medicine.medical_specialty
Histology
Angiogenesis Factor
Biology
Sensitivity and Specificity
Cell Line
Laboratory
Pathology and Forensic Medicine
Antigen-Antibody Reactions
Mice
Cytokeratin
Neoplasms
Diagnosis
Econometric and Statistical Methods: Special Topics
Preventive Health Services
Keratin
Biomarkers
Tumor
medicine
Carcinoma
Animals
Humans
Tumor pathology
Neoplasm Metastasis
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
chemistry.chemical_classification
Mice
Inbred BALB C
Special Topics [Econometric and Statistical Methods]
Antibodies
Monoclonal
Reproducibility of Results
Epithelial Cells
General Medicine
Tumor pathologie
medicine.disease
Immunohistochemistry
Diagnosis
Laboratory
Epithelium
Staining
medicine.anatomical_structure
chemistry
Keratin 7
Keratin 8
Keratins
Plasmacytoma
Zdroj: Histopathology, 31, 503-516
Histopathology, 31, pp. 503-516
Histopathology, 31, 6, pp. 503-516
ISSN: 1365-2559
0309-0167
DOI: 10.1046/j.1365-2559.1997.2960896.x
Popis: Aims: In this study the recently developed keratin 19 antibody RCK108 is biochemically and immunohistochemically characterized. Its applicability as a keratin marker in routinely processed histological tissue specimens is assessed. Methods and results: The keratin 19 antibody RCK108 antibody was tested on normal and malignant routinely formalin-fixed, paraffin-embedded tissue specimens. It stains most, although not all, glandular epithelia and showed (focal) reactivity in the basal cell compartment of stratified epithelia. It was found to react with most epithelial tumours, including adenocarcinomas, squamous cell carcinomas and endocrine tumours of various origins. Conclusions: Its reproducible and highly sensitive staining characteristics make RCK108 a useful antibody to be applied as a broad epithelial marker for carcinoma detection in routinely processed paraffin sections. As such, RCK108 is a specific reagent for practically all epithelial tumours. A few types of epithelial malignancies, known not to contain keratin 19, were negative for RCK108. Therefore the antibody is also useful in some narrow differential diagnostic considerations such as cholangiocellular carcinoma (RCK108 positive) vs. hepatocellular carcinoma (RCK108 negative). Another important feature of this antibody is that it shows very little reactivity in mesenchymal tissues, or mesenchymally derived tumours, as is frequently described for other keratin antibodies. A few leiomyosarcomas showed sporadic reactivity.
Databáze: OpenAIRE
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