Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy
| Autor: | Jian-Min Bian, Dian-Hua Gu, Ya-Bin Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Time Factors medicine.medical_treatment Blood sugar Cord Blood Stem Cell Transplantation Biology Mesenchymal Stem Cell Transplantation Umbilical cord Diabetes Mellitus Experimental Umbilical Cord Rats Sprague-Dawley Pancreatectomy In vivo Internal medicine Insulin-Secreting Cells parasitic diseases medicine Animals Humans cardiovascular diseases Wharton Jelly Cell Shape Cells Cultured Cell Proliferation Glucose tolerance test medicine.diagnostic_test C-Peptide Insulin Body Weight Gastroenterology Cell Differentiation Mesenchymal Stem Cells General Medicine Basic Study Glucose Tolerance Test Transplantation medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 1 Gene Expression Regulation |
| Popis: | AIM: To investigate the effects of transplantation of insulin-producing cells (IPCs) in the treatment of diabetic rats after 90% pancreatectomy. METHODS: Human umbilical cord mesenchymal stem cells (UCMSCs) were isolated and induced into IPCs using differentiation medium. Differentiated cells were examined by dithizone (DTZ) staining, reverse transcription-polymerase chain reaction (RT-PCR), and real-time RT-PCR. C-peptide release, both spontaneously and after glucose challenge, was measured by ELISA. IPCs were then transplanted into Sprague-Dawley rats after 90% pancreatectomy and blood glucose levels and body weight were measured. RESULTS: The differentiated cells were positive for DTZ staining and expressed pancreatic β-cell related genes. C-peptide release by the differentiated cells increased after glucose challenge (380.6 ± 15.32 pmol/L vs 272.4 ± 15.32 pmol/L, P < 0.05). Further, in the cell transplantation group, blood sugar levels were significantly lower than in the sham group 2 wk after transplantation (18.7 ± 2.5 mmol/L vs 25.8 ± 1.25 mmol/L, P < 0.05). Glucose tolerance tests showed that 45 min after intraperitoneal glucose injection, blood glucose levels were significantly lower on day 56 after transplantation of IPCs (12.5 ± 4.7 mmol/L vs 42.2 ± 9.3 mmol/L, P < 0.05). CONCLUSION: Our results show that UCMSCs can differentiate into islet-like cells in vitro under certain conditions, which can function as IPCs both in vivo and in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|