Hypoxemia modifies circulating and exudate neutrophil number and functional responses in carrageenin-induced pleurisy in the rat
| Autor: | Sylvie Marleau, D M Macari, D Martel, Christina Barja-Fidalgo, P B Tremblay, P. du Souich |
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| Rok vydání: | 2000 |
| Předmět: |
Exudate
Lipopolysaccharides Male medicine.medical_specialty Neutrophils Immunology Stimulation Carrageenan Hypoxemia Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Leukocyte Count In vivo Superoxides Internal medicine medicine Immunology and Allergy Animals Hypoxia Pleurisy Nitrites Superoxide business.industry hemic and immune systems Cell Biology medicine.disease Rats N-Formylmethionine Leucyl-Phenylalanine Endocrinology chemistry Room air distribution medicine.symptom business |
| Zdroj: | Journal of leukocyte biology. 67(6) |
| ISSN: | 0741-5400 |
| Popis: | To assess the effect of hypoxemia on the responses of polymorphonuclear neutrophils (PMN) during an inflammatory response, rats were maintained in a low FiO2 atmosphere (9% O2) or room air for 12 h before intrathoracic injection of carrageenin or intradermal injections of agonists. After 4 h, hypoxemic rats had 50% more circulating PMN in blood and 25% less PMN in pleural exudate, whereas the number of PMN in skin biopsies did not differ from controls. Following hypoxemia, basal adhesion of blood PMN to serum-coated plastic wells was unchanged, whereas fMLP-stimulated adhesion was 50% greater. In contrast, basal adhesion of exudate PMN was 72% greater. In hypoxemic rats, exudate PMN produced 64% more PMA-stimulated superoxide than blood PMN; furthermore, blood and exudate PMN produced 4.5- and 2-fold more LPS-stimulated nitric oxide than controls, respectively. These results show that a moderate level of hypoxemia may trigger mechanisms that will interfere with PMN emigration yet prime these cells for enhanced responses upon stimulation. J. Leukoc. Biol. 67: 785–792; 2000. |
| Databáze: | OpenAIRE |
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