Banff 2019 Meeting Report: Molecular diagnostics in solid organ transplantation–Consensus for the Banff Human Organ Transplant (B-HOT) gene panel and open source multicenter validation

Autor: Ivy A. Rosales, Anthony J. Demetris, Jan H. von der Thüsen, Rex Neal Smith, Jean-Paul Duong Van Huyen, Enver Akalin, Robert B. Colvin, Kathryn J. Wood, Michael Mengel, Maarten Naesens, Candice Roufosse, Alexandre Loupy, Mark Haas, Jessy Dagobert, Fadi Issa, Alberto Sanchez-Fueyo, Benjamin Adam, Marian C. Clahsen-van Groningen, Juliette Gueguen, Blaise Robin
Přispěvatelé: Pathology, Imperial College Healthcare NHS Trust- BRC Funding, Imperial Health Charity, The Pathological Society Visiting Fellowship (grant reference 1077)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Graft Rejection
diagnostic techniques and imaging
classification systems: Banff classification
Microarray
Biopsy
030230 surgery
Meeting Report
Kidney
Organ transplantation
Transcriptome
Meeting Reports
0302 clinical medicine
Gene panel
Reference genes
Immunology and Allergy
Pharmacology (medical)
Pathology
Molecular
11 Medical and Health Sciences
MICROARRAY DIAGNOSIS
classification systems
practice
ALLOGRAFT BIOPSIES
histopathology
biomarker
Life Sciences & Biomedicine
pathology/histopathology
EXPRESSION
medicine.medical_specialty
Consensus
BIOMARKERS
Computational biology
clinical research/practice
ANTIBODY-MEDIATED REJECTION
CLASSIFICATION
ISCHEMIA-REPERFUSION
03 medical and health sciences
Banff classification
INJURY
medicine
Humans
ACUTE CELLULAR REJECTION
KIDNEY-TRANSPLANTS
Transplantation
Science & Technology
business.industry
Organ Transplantation
Molecular diagnostics
Kidney Transplantation
Clinical trial
Open source
clinical research
Surgery
pathology
business
Zdroj: American Journal of Transplantation, 20(9), 2305-2317. Wiley-Blackwell Publishing Ltd
American Journal of Transplantation
ISSN: 1600-6135
Popis: This meeting report from the XV Banff conference describes the creation of a multiorgan transplant gene panel by the Banff Molecular Diagnostics Working Group (MDWG). This Banff Human Organ Transplant (B‐HOT) panel is the culmination of previous work by the MDWG to identify a broadly useful gene panel based on whole transcriptome technology. A data‐driven process distilled a gene list from peer‐reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, and lung transplant biopsies. These were supplemented by genes that define relevant cellular pathways and cell types plus 12 reference genes used for normalization. The 770 gene B‐HOT panel includes the most pertinent genes related to rejection, tolerance, viral infections, and innate and adaptive immune responses. This commercially available panel uses the NanoString platform, which can quantitate transcripts from formalin‐fixed paraffin‐embedded samples. The B‐HOT panel will facilitate multicenter collaborative clinical research using archival samples and permit the development of an open source large database of standardized analyses, thereby expediting clinical validation studies. The MDWG believes that a pathogenesis and pathway based molecular approach will be valuable for investigators and promote therapeutic decision‐making and clinical trials.
This Banff meeting report summarizes the progress of the Banff Molecular Diagnostics Working Group, which generated consensus on a transplant‐specific discovery gene panel and a potential roadmap for its validation for diagnostic application.
Databáze: OpenAIRE
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