The relationship between size of a presecretory protein and extent of signal-recognition-particle-mediated arrest of its translation
| Autor: | Richard Zimmermann, C. Jung |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Signal recognition particle
Biophysics Translation (biology) Cell Biology Biology Biochemistry Phenotype Ribosome Melitten Cell biology Globins Prepromelittin Molecular Weight Insect Hormones Protein Biosynthesis Animals Insect Proteins RNA Messenger Protein Precursors Molecular Biology Signal Recognition Particle Ribonucleoprotein |
| Zdroj: | Biochemical and biophysical research communications. 205(1) |
| ISSN: | 0006-291X |
| Popis: | Small eukaryotic presecretory proteins, such as preprocecropinA, prepromelittin, and prepropeptideGLa, are transported into mammalian microsomes both with the aid of ribosome and signal recognition particle (SRP) and independently of these ribonucleoprotein particles. Typically, synthetic extensions of these precursor proteins show the phenotype of naturally occuring large presecretory proteins. However, it was shown that small truncated forms of naturally occuring large presecretory proteins are not transport competent or transport competent only in the presence of the two ribonucleoprotein particles. In order to directly address this apparent paradox, we studied the sensitivities of nascent polypeptide chains, related to preprocecropinA and prepromelittin, to SRP-mediated arrest of elongation and compared them with synthetic extensions of various length. |
| Databáze: | OpenAIRE |
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