Mutational changes in physiochemical cell surface properties of plant-growth-stimulating Pseudomonas spp. do not influence the attachment properties of the cells
| Autor: | H. E. Klaassen, Ben J. J. Lugtenberg, L.A. de Weger, M.C.M. van Loosdrecht |
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| Rok vydání: | 2016 |
| Předmět: |
Gel electrophoresis
Lipopolysaccharides Antigens Bacterial biology Sodium Mutant Pseudomonas chemistry.chemical_element Adhesion Plants biology.organism_classification Microbiology Bacterial Adhesion Biochemistry chemistry Solubility Pseudomonadales Antigens Surface Bacteriophages Molecular Biology Bacteria Pseudomonadaceae Research Article |
| Zdroj: | Journal of Bacteriology, 171(5), 2756-2761 Journal of Bacteriology Scopus-Elsevier |
| Popis: | Bacteriophage-resistant mutant strains of the root-colonizing Pseudomonas strains WCS358 and WCS374 lack the O-antigenic side chain of the lipopolysaccharide, as was shown by the loss of the typical lipopolysaccharide ladder pattern after analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These strains differed from their parent strains in cell surface hydrophobicity and in cell surface charge. The observed variation in these physicochemical characteristics could be explained by the differences in sugar composition. The mutant strains had no altered properties of adherence to sterile potato roots compared with their parental strains, nor were differences observed in the firm adhesion to hydrophilic, lipophilic, negatively charged, or positively charged artificial surfaces. These results show that neither physicochemical cell surface properties nor the presence of the O-antigenic side chain plays a major role in the firm adhesion of these bacterial cells to solid surfaces, including potato roots. |
| Databáze: | OpenAIRE |
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