Methylation profile and amplification of proto-oncogenes in rat pancreas induced with phytoestrogens
| Autor: | P. W. Payne, E. Blann, Daniel M. Sheehan, Kevin L. Medlock, Beverly Lyn-Cook, J. Bo |
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| Rok vydání: | 1995 |
| Předmět: |
endocrine system
medicine.medical_specialty Coumestrol Estrogen receptor Phytoestrogens Biology medicine.disease_cause Methylation Proto-Oncogene Mas General Biochemistry Genetics and Molecular Biology Deoxyribonuclease HpaII Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Estrogens Non-Steroidal Estrogen binding Chromans Deoxyribonucleases Type II Site-Specific Pancreas Cells Cultured Gene Amplification food and beverages Equol DNA Molecular biology Isoflavones Rats Endocrinology Genes ras chemistry Animals Newborn DNA methylation Female Plant Preparations Carcinogenesis |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 208(1) |
| ISSN: | 0037-9727 |
| Popis: | Specific gene hypermethylation has been shown in DNA from neonatal rats exposed to the phytoestrogens, coumestrol, and equol. The pancreas is an organ in which estrogen receptors have been shown to be present. Studies have correlated the development of acute pancreatitis with rising levels of human estrogen binding proteins. Neonatal rats were dosed with 10 or 100 {mu}g of coumestrol or equol on postnatal day (PND) 1-10. The animals were sacrificed at Day 15. The pancreas was excised and pancreatic acinar cells isolated for molecular analysis. DNA was isolated from the cells by lysis in TEN-9 buffer supplemented with proteinase K and 0.1% SDS. High molecular weight (HMW) DNA was digested with the methylated DNA specific restriction enzymes, Hpa II and Msp I, for determination of methylation profiles. Both coumestrol and equol at high doses caused hypermethylation of the c-H-ras proto-oncogene. No hypermethylation or hypomethylation was observed in the proto-oncogenes, c-myc or c-fos. Methylation is thought to be an epigenetic mechanism involved in the activation (hypomethylation) or inactivation (hypermethylation) of cellular genes which are known to play a role in carcinogenesis. Epidemiology studies have shown that equol may have anti-carcinogenic effects on some hormone-dependent cancers. Additional studies are needed tomore » further understand the role of phytoestrogens and methylation in relation to pancreatic disorders. 15 refs., 4 figs.« less |
| Databáze: | OpenAIRE |
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