CD8+ T cells in situ in different clinical forms of human cutaneous leishmaniasis
| Autor: | Lucas P. Carvalho, Sara Passos, Marina Loyola Dantas, Adriano Queiroz, Edgar M. Carvalho, Paulo Roberto Lima Machado, Sérgio Arruda, Juliana Menezes Gomes Cabral de Oliveira |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Microbiology (medical) Pathology medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 Cytotoxicity Leishmaniasis Cutaneous CD8-Positive T-Lymphocytes Granzymes Article Lesion Young Adult Cutaneous leishmaniasis medicine Humans Cytotoxic T cell Child Leishmaniasis Inflammation biology Papillary dermis CD8 Middle Aged medicine.disease biology.organism_classification Immunohistochemistry Leishmania braziliensis Granzyme B Cutaneous Infectious Diseases Immunology Female Parasitology medicine.symptom Human |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 6, Pp 728-734 (2013) Revista da Sociedade Brasileira de Medicina Tropical, Volume: 46, Issue: 6, Pages: 728-734, Published: DEC 2013 Revista da Sociedade Brasileira de Medicina Tropical v.46 n.6 2013 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| ISSN: | 0037-8682 |
| DOI: | 10.1590/0037-8682-0174-2013 |
| Popis: | Introduction Leishmania braziliensis infection induces a large spectrum of lesions that clinically manifest as nodules or papules that progress to ulcers. Although it is already known that T helper cells predominate in the lesions, cytotoxic T cells have also been reported to be present, and their role in leishmaniasis immunopathogenesis is not well known. This study investigated the amounts of CD8+ and granzyme B+ cells in different clinical forms of human cutaneous leishmaniasis (CL). Methods Forty tissue fragments from early (E-CL) and late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and ulcers - were characterized. The inflamed area per fragment was calculated, and the CD8 and granzyme B expression levels in the infiltrates were quantified by counting positive cells in 15 fields. The localization of CD8 and granzyme B was graded subjectively. Results Inflammation was higher in L-CL and DL ulcers. CD8 expression was increased in late ulcerated lesions compared to recent lesions. The increase in CD8+ cells also correlated with the duration of the lesion. Papules had a higher frequency of granzyme B+ cells than E-CL lesions, although the frequency was similar to those for late and DL ulcers. CD8+ cells were mostly found in the papillary dermis. Conclusions CD8+ T and granzyme B+ cells are present in the inflammatory infiltrates of CL and DL and may participate in the immunopathogenesis of Leishmania infection. |
| Databáze: | OpenAIRE |
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