In situ microfluidic SERS assay for monitoring enzymatic breakdown of organophosphates
| Autor: | Peter A. Carr, Kimberly Hamad-Schifferli, Scott T. Wick, Todd Thorsen, Vladimir Liberman |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Microscope
Silver Ethanethiol Microfluidics Analytical chemistry Metal Nanoparticles Photochemistry Spectrum Analysis Raman law.invention symbols.namesake chemistry.chemical_compound law Hydrolase General Materials Science chemistry.chemical_classification Chemistry Aryldialkylphosphatase Equipment Design Surface-enhanced Raman spectroscopy Microfluidic Analytical Techniques Organophosphates Raman laser symbols Thiol Raman spectroscopy Biotechnology |
| Zdroj: | Nanoscale. 7(25) |
| ISSN: | 2040-3372 |
| Popis: | In this paper, we report on a method to probe the breakdown of the organophosphate (OP) simulants o,s-diethyl methyl phosphonothioate (OSDMP) and demeton S by the enzyme organophosphorous hydrolase (OPH) in a microfluidic device by surface enhanced Raman spectroscopy (SERS). SERS hotspots were formed on-demand inside the microfluidic device by laser-induced aggregation of injected Ag NPs suspensions. The Ag NP clusters, covering micron-sized areas, were formed within minutes using a conventional confocal Raman laser microscope. These Ag NP clusters were used to enhance the Raman spectra of the thiol products of OP breakdown in the microfluidic device: ethanethiol (EtSH) and (ethylsulfanyl) ethane-1-thiol (2-EET). When the OPH enzyme and its substrates OSDMP and demeton S were introduced, the thiolated breakdown products were generated, resulting in changes in the SERS spectra. With the ability to analyze reaction volumes as low as 20 nL, our approach demonstrates great potential for miniaturization of SERS analytical protocols. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|