Integration of galacturonic acid extraction with alkaline protein extraction from green tea leaf residue
| Autor: | Marieke E. Bruins, Frits van der Klis, Yiyuan Dong, Elvira Bozileva, Johan P.M. Sanders, Chen Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Cost estimation food.ingredient Pectin Biobased Chemistry and Technology Cellulase Raw material 01 natural sciences 0404 agricultural biotechnology food 010608 biotechnology BBP Sustainable Chemistry & Technology Protein purification Biobased Products Recycle system VLAG Residue (complex analysis) Chromatography biology Chemistry Extraction (chemistry) 04 agricultural and veterinary sciences Biorefinery Leafy biomass 040401 food science Experimental design Yield (chemistry) biology.protein Food Technology Agronomy and Crop Science |
| Zdroj: | Industrial Crops and Products, 89, 95-102 Industrial Crops and Products 89 (2016) |
| ISSN: | 0926-6690 |
| Popis: | Leaf pectin can be used as a feedstock for galacturonic acid (GA) production, but high extraction costs limit economic feasibility. To improve the extraction efficiency, leaf pectin extraction was integrated with an already cost-effective alkaline protein extraction, focusing on high yield of GA without losses of protein. GA extraction efficiencies in NaOH, HCl, phosphate buffer solution, or with Viscozyme® L were determined using green tea residues (GTR) as model material. Most GA was extracted using Viscozyme® L, mainly due to its cellulase activity. Extraction yielded more than 95% GA with only 5% protein. Alternatively, GA-containing pectin can be extracted in a weak alkaline solution. Here, GA yield is dominated by the ratio of extraction volume to biomass weight. The profits of these two integrated processes can be higher than one step protein extraction. The Viscozyme® L integrated process is suitable for GA production for application in chemicals, and may have a profit of 142$/ton GTR when enzyme cost are sufficiently lowered. The profit of the weak alkaline integrated process is estimated at 118$/ton GTR. |
| Databáze: | OpenAIRE |
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