Phospholipid composition of membranes in the tumor cell
| Autor: | Gor'kova Np, Sorokina Ib, Ella V. Dyatlovitskaya, T.I. Torkhovskaya, Lev D. Bergelson |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Male
Carcinoma Hepatocellular Chromatography Paper Biophysics Phospholipid Mitochondria Liver Biology Mitochondrion Biochemistry law.invention Mice chemistry.chemical_compound Endocrinology law Organelle Animals Hepatectomy Phospholipids Cell Nucleus Phosphatidylethanolamines Cell Membrane Fatty Acids Liver Neoplasms Lysophosphatidylcholines Phosphorus Cell Fraction Liver Regeneration Rats Sphingomyelins Microscopy Electron Paper chromatography Membrane chemistry Microsomes Liver Phosphatidylcholines Microsome Female Chromatography Thin Layer Sarcoma Experimental Electron microscope |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 210:287-298 |
| ISSN: | 0005-2760 |
| Popis: | Mitochondria, microsomes, nuclei and plasma membranes of normal liver and various hepatomas were isolated and their purity characterized by electron microscopy as well as by the absence of marker enzymes. The individual phospholipids of the subcellular fractions were identified by their chromatographic behavior and by paper chromatography of their deacylation products and determined quantitatively by their phosphorus content. It was found that the specific phospholipid distribution characteristic of subcellular particles of normal liver no longer occurs in organelles isolated from hepatoma cells. In the latter the phospholipid patterns of the different membrane types are equalized and in some cases become almost identical. Since from control experiments it seems unlikely that complete redistribution of the lipids could take place during the isolation of cell fractions, the results obtained reveal a trend to chemical dedifferentiation of the membranes in the hepatoma cell. The same trend is observed in other types of experimental tumors but not in regenerating rat liver and hence do not appear to be connected with rapid growth per se. |
| Databáze: | OpenAIRE |
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