An oviducal enzyme isolated by affinity chromatography which acts upon the vitelline envelope of Bufo arenarum coelomic oocytes
| Autor: | Enrique J. del Pino, Dora C. Miceli, Silvia N. Fernández |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Chromatography
biology Size-exclusion chromatography Vitelline membrane General Medicine Oviducts Chromatography Affinity Sepharose Affinity chromatography Biochemistry Concanavalin A Sephadex Endopeptidases biology.protein Bufo arenarum Oocytes Animals Female Trypsin Polyacrylamide gel electrophoresis Vitelline Membrane Ovum |
| Zdroj: | Biochimica et biophysica acta. 526(1) |
| ISSN: | 0006-3002 |
| Popis: | A trypsin-like oviducal proteinase acting upon the vitelline envelope of Bufo arenarum coelomic oocytes has been purified to apparent homogeneity by gel filtration on Sephadex G-200 and by affinity chromatography on a column of Sepharose 4-B containing covalently bound concanavalin A (Con A). The biologically active molecule migrated as a single band of protein upon SDS polyacrylamide gel electrophoresis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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