Long-Chain Non-Coding RNA (lncRNA) MT1JP Suppresses Biological Activities of Lung Cancer by Regulating miRNA-423-3p/Bim Axis
| Autor: | Haijun Yan, Ji-Yong Ma, Jing Zhang, Yan Tan, Wei Gu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Lung Neoplasms Cell Apoptosis 030204 cardiovascular system & hematology Ankle Fractures Flow cytometry 03 medical and health sciences 0302 clinical medicine Lab/In Vitro Research Cell Movement Carcinoma Non-Small-Cell Lung microRNA medicine Humans Neoplasm Invasiveness Cell Proliferation A549 cell Bcl-2-Like Protein 11 medicine.diagnostic_test Chemistry Cell growth Cancer General Medicine Middle Aged medicine.disease Blot MicroRNAs medicine.anatomical_structure A549 Cells 030220 oncology & carcinogenesis Cancer research RNA Long Noncoding Female Metallothionein |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | strongBACKGROUND/strongThis study aimed to explain the effects and mechanism of MT1JP in lung cancer development and treatment.strongMATERIAL AND METHODS/strongThirty non-small cell lung cancer (NSCLC) (stages I-II, 17 cases; stages III-IV, 13 cases) and adjacent normal tissues were obtained. MT1JP and miRNA-423-3p levels were assessed byiin situ/ihybridization and Bim protein expression by immunohistochemistry, and the correlations determined were analyzed. Cell proliferation was determined using MTT and colony formation assay, and cell apoptosis was measured using flow cytometry. A549 cell invasion and migration were assessed by Transwell migration and scratch wound healing assays. Relative mRNA and protein expressions were assessed using real-time polymerase chain reaction and western blotting. Correlations between miRNA-423-3p and Bim protein were investigated using luciferase activity assay, and Bim protein expression was evaluated using western blotting.strongRESULTS/strongMT1JP, miRNA-423-3p, and Bim expressions in NSCLC cancer tissues and those in adjacent cancer tissues were significantly different (iP/i0.01 oriP/i0.001) with increasing stage. Compared with those in the normal control (NC) group, cell proliferation rates were significantly suppressed (iP/i0.01 oriP/i0.001) and cell apoptosis rates significantly increased (iP/i0.01 oriP/i0.001) in the miRNA inhibitor and lncRNA+miRNA inhibitor groups. Invasion cell numbers and wound healing rates were also significantly inhibited in the miRNA inhibitor and lncRNA+miRNA inhibitor groups (iP/i0.01 oriP/i0.001) compared with those in the NC group.strongCONCLUSIONS/strongThe lncRNA MT1JP suppresses NSCLC biological activities by regulating the miRNA-423-3p/Bim axis. |
| Databáze: | OpenAIRE |
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