Enteropathogen infections in canine puppies: (Co-)occurrence, clinical relevance and risk factors

Autor: Duijvestijn, Mirjam, Mughini-Gras, Lapo, Schuurman, Nancy, Schijf, Wim, Wagenaar, Jaap A, Egberink, Herman, dI&I I&I-1, LS Klinisch Onderzoek Wagenaar, LS Virologie, dI&I I&I-4, Infection & Immunity
Přispěvatelé: dI&I I&I-1, LS Klinisch Onderzoek Wagenaar, LS Virologie, dI&I I&I-4, Infection & Immunity
Rok vydání: 2016
Předmět:
Gastro-enteritis
0301 basic medicine
Veterinary medicine
Clinical score
Gastrointestinal Diseases
040301 veterinary sciences
Cystoisospora
viruses
animal diseases
Determinants of infection
030106 microbiology
Breeding
medicine.disease_cause
Microbiology
Article
0403 veterinary science
03 medical and health sciences
Dogs
Enteropathogens
Risk Factors
medicine
Animals
Dog Diseases
Feces
Netherlands
Host Pathogen Interaction & Diagnostics
General Veterinary
biology
Campylobacter
Bacteriologie
Canine parvovirus
Giardia
Bacteriology
Canine coronavirus
Bacteriology
Host Pathogen Interaction & Diagnostics
Bacterial Infections
04 agricultural and veterinary sciences
General Medicine
Clostridium difficile
biology.organism_classification
veterinary(all)
Virology
Diarrhoea
Host Pathogen Interactie & Diagnostiek
Co-infection
Virus Diseases
Bacteriologie
Host Pathogen Interactie & Diagnostiek
GIARDIA SPP
Zdroj: Veterinary Microbiology
Veterinary Microbiology 195 (2016)
Veterinary Microbiology, 195, 115-122
Veterinary Microbiology, 195, 115. Elsevier
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2016.09.006
Popis: Highlights • CPV, CCoV and β-hemolytic E. coli are associated with (severe) acute diarrhoea in puppies. • Puppies from high-volume breeders are more likely to be infected with CPV, CCoV and Cystoisospora spp. • CPV and CCoV are more often detected in winter/spring. • Multiple enteric infections are common in puppies with and without diarrhoea. • Screening of CPV, CCoV and β-hemolytic E. coli is advised in diarrhoeic puppies.
Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. Faecal samples from puppies with (n = 113) and without (n = 56) acute diarrhoea were collected and screened for Canine Parvovirus (CPV), Canine Coronavirus (CCoV), Salmonella spp., Campylobacter spp., Clostridium perfringens, Clostridium difficile, β-hemolytic Eschericha coli (hEC), Giardia spp., Toxocara spp., Cystoisospora spp., and Cyniclomyces guttulatus. One or more pathogens were detected in 86.5% of diarrhoeic puppies and in 77.8% of asymptomatic puppies. Significant positive associations were found between CPV and CCoV, CPV and Cystoisospora spp., Toxocara spp. and hEC, Giardia spp. and C. guttulatus. Only CPV and CCoV were significantly associated with diarrhoea, hEC with a subset of puppies that had diarrhoea and severe clinical signs. CPV was more prevalent in puppies under 3 months of age. Puppies from high-volume dog breeders were significantly at increased risk for CPV (OR 4.20), CCoV (OR 4.50) and Cystoisospora spp. (OR 3.60). CCoV occurred significantly more often in winter (OR 3.35), and CPV in winter (OR 3.78) and spring (OR 4.72) as compared to summer. We conclude that routine screening for CPV, CCoV and hEC is recommended in puppies with acute diarrhoea, especially if they are under 3 months of age and originate from high-volume dog breeders. Routine screening for other pathogens may lead to less conclusive results.
Databáze: OpenAIRE
Externí odkaz: