Enlisting the Ixodes scapularis Embryonic ISE6 Cell Line to Investigate the Neuronal Basis of Tick—Pathogen Interactions

Autor: Lourdes Mateos-Hernández, Anne-Claire Lagrée, Clotilde Rouxel, Céline Henry, Natália Pipová, José de la Fuente, Henri-Jean Boulouis, Pilar Alberdi, Eleonore Allain, James J. Valdés, Ladislav Šimo, Nadia Haddad, Alejandro Cabezas-Cruz
Přispěvatelé: Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Pavol Jozef Šafárik, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Parasitology [Ceske Budejovice, Czech Republic], Biology Centre of the Czech Academy of Sciences (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Instituto de Investigación en Recursos Cinegéticos (IREC), Regional Centre for Biomedical Research ((CRIB)), University of Castilla-La Mancha (UCLM), Department of Veterinary Pathobiology, Center of Veterinary Health Sciences, Oklahoma State University [Stillwater], DIM1Health—Région Île-de-France (Acronym of the project: NeuroPaTick), Purchase of confocal microscopy used in this study was supported by the DIM1Health—Région Île-de-France (Acronym of the project: SAMiCAI)., French Institute of Slovakia/French embassy of Slovakia, Project FIT (Pharmacology, Immunotherapy, nanoToxicology), which was funded by the European Regional Development Fund and acknowledge a grant for the development of a research organization RVO: RO0516., Région Ile-de-France, European Commission, École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Pathogens
Pathogens, MDPI, 2021, 10 (1), pp.1-15. ⟨10.3390/pathogens10010070⟩
Volume 10
Issue 1
Digital.CSIC. Repositorio Institucional del CSIC
instname
Pathogens, Vol 10, Iss 70, p 70 (2021)
ISSN: 2076-0817
Popis: This article belongs to the Section Ticks.
Neuropeptides are small signaling molecules expressed in the tick central nervous system, i.e., the synganglion. The neuronal-like Ixodes scapularis embryonic cell line, ISE6, is an effective tool frequently used for examining tick–pathogen interactions. We detected 37 neuropeptide transcripts in the I. scapularis ISE6 cell line using in silico methods, and six of these neuropeptide genes were used for experimental validation. Among these six neuropeptide genes, the tachykinin-related peptide (TRP) of ISE6 cells varied in transcript expression depending on the infection strain of the tick-borne pathogen, Anaplasma phagocytophilum. The immunocytochemistry of TRP revealed cytoplasmic expression in a prominent ISE6 cell subpopulation. The presence of TRP was also confirmed in A. phagocytophilum-infected ISE6 cells. The in situ hybridization and immunohistochemistry of TRP of I. scapularis synganglion revealed expression in distinct neuronal cells. In addition, TRP immunoreaction was detected in axons exiting the synganglion via peripheral nerves as well as in hemal nerve-associated lateral segmental organs. The characterization of a complete Ixodes neuropeptidome in ISE6 cells may serve as an effective in vitro tool to study how tick-borne pathogens interact with synganglion components that are vital to tick physiology. Therefore, our current study is a potential stepping stone for in vivo experiments to further examine the neuronal basis of tick–pathogen interactions.
This research was funded by DIM1Health—Région Île-de-France (Acronym of the project: NeuroPaTick), the purchase of confocal microscopy used in this study was supported by the DIM1Health—Région Île-de-France (Acronym of the project: SAMiCAI). The NP was funded by French Institute of Slovakia/French embassy of Slovakia, the JJV was supported by Project FIT (Pharmacology, Immunotherapy, nanoToxicology), which was funded by the European Regional Development Fund and acknowledge a grant for the development of a research organization RVO: RO0516.
Databáze: OpenAIRE
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