Characteristics of extended-spectrum β-lactamase (ESBL)- and pAmpC beta-lactamase-producing Enterobacteriaceae of water samples in Tunisia

Autor: Karim Ben Slama, Carla Andrea Alonso, Raoudha Dziri, Leila Ben Said, Ahlem Jouini, Abdellatif Boudabous, Naouel Klibi, Carmen Torres
Přispěvatelé: Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM), Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire (LR11IPT03), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Universidad de La Rioja (UR), Institut supérieur des sciences biologiques appliquées de Tunis = Higher Institute of Applied Biological Sciences of Tunis (ISSBAT), This work was partially supported by Project SAF2012-35474 from the Ministerio de Economía y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER), and by a project from the Tunisian Ministry of Higher Education, Scientific Research and Information and Communication Technologies., We thank A. Caratolli for providing us with positive controls for PBRT testing.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Klebsiella pneumoniae
medicine.medical_treatment
MESH: Enterobacteriaceae/genetics
010501 environmental sciences
Wastewater
medicine.disease_cause
01 natural sciences
MESH: Bacterial Proteins/metabolism
Plasmid
CTX-M
MESH: Environmental Monitoring
Waste Management and Disposal
MESH: Water Microbiology
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
biology
Strain (chemistry)
Pollution
Enterobacteriaceae
6. Clean water
Citrobacter freundii
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: beta-Lactamases/analysis
MESH: Genes
Bacterial
Water Microbiology
MESH: Tunisia
Environmental Monitoring
Environmental Engineering
Tunisia
MESH: beta-Lactamases/metabolism
MESH: Enterobacteriaceae/growth & development
030106 microbiology
beta-Lactamases
Microbiology
03 medical and health sciences
pAmpC
Bacterial Proteins
Drug Resistance
Bacterial
MESH: Drug Resistance
Bacterial
medicine
Environmental Chemistry
Gene
Escherichia coli
0105 earth and related environmental sciences
MESH: Bacterial Proteins/analysis
biology.organism_classification
bacterial infections and mycoses
MESH: Water Pollutants
Chemical/analysis
ESBL
Genes
Bacterial
MESH: Enterobacteriaceae/metabolism
Beta-lactamase
Water Pollutants
Chemical
Zdroj: Science of the Total Environment
Science of the Total Environment, Elsevier, 2016, 550, pp.1103-1109. ⟨10.1016/j.scitotenv.2016.01.042⟩
ISSN: 0048-9697
1879-1026
Popis: International audience; The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E. coli, one Klebsiella pneumoniae, one Citrobacter freundii), blaCTX-M-14 (one E. coli) and blaCMY-2 (four E. coli). The blaTEM-1, blaOXA-1 or blaSHV-1 genes were also found in 72% of these isolates. The ISEcp1, orf477 or IS903 sequences were found upstream or downstream of blaCTX-M genes. Class 1 integrons were present in 16 of the 25 ESBL-Eb/pAmpC-Eb strains (64%), and contained five different gene-cassette arrays. Most of the strains (76%) showed a multiresistant phenotype and qnr genes were identified in four strains. Molecular typing of ESBL/CMY-2-producing E. coli isolates showed 23 different PFGE-patterns and 15 different sequence-types (ST10, ST46, ST48, ST58, ST69, ST101, ST117, ST131, ST141, ST288, ST359, ST399, ST405, ST617, and the new ST4530); these strains were ascribed to phylogroups A (11 isolates), B1 (3 isolates), D (6 isolates) and B2 (3 isolates). From one to five plasmids were detected in each strain (size from 30 kb to > 240 kb) and ESBL or pAmpC genes were transferred by conjugation in 69.5% of the E. coli strains. In conclusion, ESBL-Eb and pAmpC-Eb strains are frequently detected in wastewater samples and they might be a source for dissemination in other environments with repercussion in public health.
Databáze: OpenAIRE
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