Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus
| Autor: | Ping Han Huang, Wang Jing Liu, Li-Kwan Chang, Wei Tao Shen, Chu Fang Lo, Shu Han Yang |
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| Rok vydání: | 2012 |
| Předmět: |
Gene Expression Regulation
Viral Insecta Sp1 Transcription Factor Sequence analysis Molecular Sequence Data Immunology White spot syndrome Kruppel-Like Transcription Factors Cell Cycle Proteins Cell Line Penaeus monodon Mice Viral Proteins White spot syndrome virus 1 Penaeidae Hemolymph Complementary DNA Animals Humans Cloning Molecular Gene knockdown Expressed sequence tag Sp1 transcription factor Base Sequence biology fungi biology.organism_classification Molecular biology DNA Virus Infections Repressor Proteins Gene Knockdown Techniques Apoptosis Regulatory Proteins Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 36:121-129 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2011.06.008 |
| Popis: | Sp1-like proteins and Kruppel-like factors (KLFs) are highly related zinc-finger proteins that have crucial roles in transcription. One expressed sequence tag (EST, HPA-N-S01-EST0038) from shrimps is homologous to Sp1. This study reports the cloning and characteristics of a KLF from shrimp, Penaeus monodon (PmKLF). The full-length PmKLF cDNA is 1702 bp, encoding a polypeptide of 360 amino acids. Sequence analysis revealed that the sequence of PmKLF is similar to that of KLF11 in humans, mice and zebrafish. RT-PCR analysis indicated that PmKLF mRNA is expressed in all examined tissues. Additionally, immunofluorescence analysis revealed that GFP-KLF fusion protein is located in the nucleus as dots in an insect cell line, Sf9. Localization of PmKLF in the nucleus is also observed in the hemolymph from white spot syndrome virus (WSSV)-infected and WSSV-uninfected Litopenaeus vannamei. Knockdown of the expression of PmKLF transcript in WSSV-infected shrimp resulted in delayed cumulative mortalities, suggesting that PmKLF is important to WSSV infection. Moreover, inhibition of PmKLF expression reduced the copy number of WSSV and ie1 expression, revealing that PmKLF affects WSSV infection via interfering with ie1 expression. |
| Databáze: | OpenAIRE |
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