Epicardial Mesothelial Cells Synthesize and Release Endothelin
| Autor: | de Bold Ml, Eid H, de Bold Aj, Chen Jh |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Heart Ventricles Radioimmunoassay Cross Reactions Biology Peptide hormone Contractile protein Epithelium Rats Sprague-Dawley Internal medicine medicine Animals RNA Messenger Cells Cultured Chromatography High Pressure Liquid Pharmacology Dose-Response Relationship Drug Angiotensin II Endothelins Antibodies Monoclonal Nucleic Acid Hybridization Epithelial Cells Blotting Northern Phenotype Rats Mesothelium Microscopy Electron Endocrinology medicine.anatomical_structure Gene Expression Regulation Cell culture Circulatory system Cardiology and Cardiovascular Medicine Endothelin receptor Pericardium Cell Division Mesothelial Cell |
| Zdroj: | Journal of Cardiovascular Pharmacology. 24:715-720 |
| ISSN: | 0160-2446 |
| DOI: | 10.1097/00005344-199424050-00005 |
| Popis: | Adult rat ventricular cardiocytes, when cocultured with epicardial mesothelial cells (EMC), demonstrate remarkable plasticity of phenotype accompanied by a significant increase in cardiocyte contractile protein content, suggesting that a factor with growth-promoting properties may take part in EMC-adult rat ventricular cardiocyte interactions. Endothelin (ET) has been shown to induce cell hypertrophy, including enhancement of expression of muscle-specific genes. We investigated the ability of EMC to synthesize and release ET. By light microscopy, specific immunostaining, with either ET-1 or Big ET-1 antibodies, was visualized in EMC as a fine punctate distributed throughout the cytoplasm. Reverse phase-high performance liquid chromatography (HPLC) of epicardial mesothelial cells conditioned medium showed several peaks of immunoreactive ET. The major peak eluted with the same retention time as that of ET-1. By Northern blot analysis, a specific 2.3-kilobase (kb) mRNA species was detected by hybridization to a cDNA insert encoding for rat prepro-ET-1. ET accumulated in the culture medium in a time-dependent manner, whereas cell content remained comparatively low. Angiotensin II (AII) dose-dependently stimulated release of immunoreactive ET into the culture medium. |
| Databáze: | OpenAIRE |
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