An improved procedure for isolating adult mouse cardiomyocytes for epicardial activation mapping
| Autor: | Zichao Hu, Dehua He, Zhengrong Huang, Ziguan Zhang, Qiang Xie, Meirong Huang, Weihua Li, Wuyang Zheng |
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| Rok vydání: | 2021 |
| Předmět: |
Genetically modified mouse
Epicardial Mapping Electroanatomic mapping Patch-Clamp Techniques Cell Culture Techniques Drug Evaluation Preclinical Action Potentials adult mouse cardiomyocytes Cell Separation Disease pathogenesis Mice In vivo Medicine Animals Myocytes Cardiac Patch clamp epicardial activation mapping Enzymatic digestion business.industry Retrograde intubation cardiomyocyte isolation Langendorff Cell Biology Original Articles electrophysiology Cell biology Molecular Medicine Original Article business Electrophysiologic Techniques Cardiac Perfusion |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 |
| Popis: | Cardiovascular disease is a leading cause of death and disability worldwide. Although genetically modified mouse models offer great potential for robust research in vivo, in vitro studies using isolated cardiomyocytes also provide an important approach for investigating the mechanisms underlying cardiovascular disease pathogenesis and drug actions. Currently, isolation of mouse adult cardiomyocytes often relies on aortic retrograde intubation under a stereoscopic microscope, which poses considerable technical barriers and requires extensive training. Although a simplified, Langendorff‐free method has been used to isolate viable cardiomyocytes from the adult mouse heart, the system requires enzymatic digestions and continuous manual technical operation. This study established an optimized approach that allows isolation of adult mouse cardiomyocytes and epicardial activation mapping of mouse hearts using a Langendorff device. We used retrograde puncture through the abdominal aorta in vivo and enzymatic digestion on the Langendorff perfusion device to isolate adult mouse cardiomyocytes without using a microscope. The yields of isolated cardiomyocytes were amenable to patch clamp techniques. Furthermore, this approach allowed epicardial activation mapping. We used a novel, simplified method to isolate viable cardiomyocytes from adult mouse hearts and to map epicardial activation. This novel approach could be beneficial in more extensive research in the cardiac field. |
| Databáze: | OpenAIRE |
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