Influence of Interleukin-10 on the Interleukin-1 Receptor Antagonist/Interleukin-1β Ratio in the Colonic Mucosa of Ulcerative Colitis
| Autor: | Kazuaki Otsuka, Kisei Ishizuka, Hitoshi Asakura, Osamu Yamaguchi, Masaaki Kobayashi, Kazunori Tashiro, Jun Matsuzawa, T. Mochizuki, Kouji Suzuki, Terasu Homma, Kazuhito Sugimura |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biology Pathogenesis medicine Humans Intestinal Mucosa Receptor Inflammation Polymorphism Genetic Gastroenterology Antagonist Receptors Interleukin-1 Interleukin Middle Aged medicine.disease Ulcerative colitis digestive system diseases Interleukin-10 Interleukin 10 Interleukin 1 receptor antagonist Cytokine Immunology Cytokines Colitis Ulcerative Female Interleukin-1 |
| Zdroj: | Digestion. 63:22-27 |
| ISSN: | 1421-9867 0012-2823 |
| Popis: | A decrease in the ratio of IL-1ra/IL-1 beta produced regionally by the colonic mucosa of patients with ulcerative colitis (UC) is believed to play a role in the pathogenesis of UC. To investigate factors influencing intramucosal IL-1ra/IL-1 beta ratios, we evaluated polymorphism of the IL-1ra gene and the production of mucosal cytokines in Japanese patients with UC. Colonic biopsy specimens of mucosal tissue were placed in organ cultures for 24 h. Then, the supernatant concentrations of IL-1 beta, IL-1ra, IL-8, IL-4, IL-10, and TGF-beta were assayed by ELISA. Genomic DNA was extracted from patient peripheral blood samples, then IL-1ra gene polymorphism was determined using PCR amplification. The mucosa from patients with active stage UC showed a tendency toward a decreased IL-1ra/IL-1 beta ratio. In the resolving stage, IL-1ra/IL-1 beta ratios increased with increasing IL-10 and TGF-beta concentrations. The addition of human recombinant IL-10 to the culture supernatants produced concentration-dependent inhibition of IL-1 beta. In Japanese patients with UC, the IL-1ra allele gene 2 phenotype had no effect on the IL-1ra/IL-1 beta ratio. Our findings suggest that a relative deficiency of IL-10 in patients with UC may contribute to persistent inflammatory changes. |
| Databáze: | OpenAIRE |
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