Improvement of microbial strains and fermentation processes
| Autor: | R. J. Strobel, V. A. Vinci, S. Parekh |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Recombination
Genetic Bacteria Emerging technologies Process (engineering) business.industry Fungi Mutagenesis (molecular biology technique) General Medicine Industrial microbiology Biology Applied Microbiology and Biotechnology Culture Media Biotechnology Industrial Microbiology Bioreactors Mutagenesis Fermentation Process optimization Biochemical engineering Selection Genetic business Screening procedures Selection (genetic algorithm) |
| Zdroj: | Applied Microbiology and Biotechnology. 54:287-301 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s002530000403 |
| Popis: | Improvement of microbial strains for the over-production of industrial products has been the hallmark of all commercial fermentation processes. Conventionally, strain improvement has been achieved through mutation, selection, or genetic recombination. Over-production of primary or secondary metabolites is a complex process, and successful development of improved strains requires a knowledge of physiology, pathway regulation and control, and the design of creative screening procedures. In addition, it requires mastery of the fermentation process for each new strain, as well as sound engineering know-how for media-optimization and the fine-tuning of process conditions. This review focuses on the various options that may be employed to improve microbial strains and addresses the complex problems of screening, the tools and technology behind the selection of targeted organisms, and the importance of process optimization. Furthermore, this review discusses new and emerging technologies and designing optimized media for tracking mutants with enhanced productivity or other desired attributes. |
| Databáze: | OpenAIRE |
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