Organ-specific ECM arrays for investigating cell-ECM interactions during stem cell differentiation
| Autor: | Joseph Candiello, Willi Halfter, Ipsita Banerjee, Mahalia Bradford, Suzanne Bertera, Saik Kia Goh, Vimal Vaidya, Thomas Richardson |
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| Rok vydání: | 2020 |
| Předmět: |
Pluripotent Stem Cells
Cellular differentiation Cell Biomedical Engineering Bioengineering Cell Communication 02 engineering and technology Biology Biochemistry Regenerative medicine Biomaterials Extracellular matrix 03 medical and health sciences Tissue engineering medicine Induced pluripotent stem cell 030304 developmental biology 0303 health sciences Decellularization Tissue Engineering Cell Differentiation General Medicine 021001 nanoscience & nanotechnology Extracellular Matrix Cell biology medicine.anatomical_structure Stem cell 0210 nano-technology Biotechnology |
| Zdroj: | Biofabrication. 13:015015 |
| ISSN: | 1758-5090 1758-5082 |
| Popis: | Pluripotent stem cells are promising source of cells for tissue engineering, regenerative medicine and drug discovery applications. The process of stem cell differentiation is regulated by multi-parametric cues from the surrounding microenvironment, one of the critical one being cell interaction with extracellular matrix (ECM). The ECM is a complex tissue-specific structure which is an important physiological regulator of stem cell function and fate. Recapitulating this native ECM microenvironment niche is best facilitated by decellularized tissue/organ derived ECM, which can faithfully reproduce the physiological environment with high fidelity to in vivo condition and promote tissue-specific cellular development and maturation. Recognizing the need for organ specific ECM in a 3D culture environment in driving phenotypic differentiation and maturation of hPSCs, we fabricated an ECM array platform using native-mimicry ECM from decellularized organs (namely pancreas, liver and heart), which allows cell-ECM interactions in both 2D and 3D configuration. The ECM array was integrated with rapid quantitative imaging for a systematic investigation of matrix protein profiles and sensitive measurement of cell-ECM interaction during hPSC differentiation. We tested our platform by elucidating the role of the three different organ-specific ECM in supporting induced pancreatic differentiation of hPSCs. While the focus of this report is on pancreatic differentiation, the developed platform is versatile to be applied to characterize any lineage specific differentiation. |
| Databáze: | OpenAIRE |
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