Depletion of abundant human serum proteins by per se imprinted cryogels based on sample heterogeneity
| Autor: | Ya-Ru Liu, Jian Wang, Li-Li Tian, Yu-Yang Wang, Wei-Qin Cao, Yan Zhang, Ya-Nan Yan, Chun Yang |
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| Rok vydání: | 2017 |
| Předmět: |
Serum
Globulin Polymers Polyacrylamide 02 engineering and technology 01 natural sciences Biochemistry Molecular Imprinting chemistry.chemical_compound Serotransferrin Humans Molecular Biology Chromatography Molecular Structure biology Chemistry 010401 analytical chemistry Molecularly imprinted polymer Albumin Blood Proteins 021001 nanoscience & nanotechnology Blood proteins 0104 chemical sciences Electrophoresis Proteome biology.protein Electrophoresis Polyacrylamide Gel 0210 nano-technology Cryogels |
| Zdroj: | PROTEOMICS. 17:1600284 |
| ISSN: | 1615-9853 |
| DOI: | 10.1002/pmic.201600284 |
| Popis: | Macroporous cryogels were prepared and used to deplete abundant proteins. It was accomplished based on the sample heterogeneity rather than any exogenous assistance. Human serum was added in monomer solutions to synthesize molecularly imprinted polymers; therein some abundant proteins were imprinted in the polyacrylamide cryogels. Meanwhile the rare components remained aqueous. Chromatography and electrophoresis showed that albumin, serotransferrin, and most globulins were depleted by columns packed with the molecularly imprinted polymers. After the depletion, lower abundance proteins were revealed by SDS-PAGE, peptide fingerprint analysis, and identified by MALDI-TOF-MS. This is an example that a "per se imprint" protocol enables to gradually dimidiate proteomes, simplify sample complexities, and facilitate further proteome profiling or biomarker discovery. |
| Databáze: | OpenAIRE |
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