Efficient enzymatic degradation process for hydrolysis activity of the Carrageenan from red algae in marine biomass
| Autor: | Dae Hee Kang, Jeong Eun Hyeon, Seung Wook Kim, Sung Ok Han, Seung Kyou You |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Glycoside Hydrolases
Chromosomal Proteins Non-Histone Recombinant Fusion Proteins Cell Cycle Proteins Bioengineering Dockerin Red algae Carrageenan Polysaccharide Applied Microbiology and Biotechnology chemistry.chemical_compound Hydrolysis Bacterial Proteins Escherichia coli Biomass Cellulose Pseudoalteromonas carrageenovora chemistry.chemical_classification biology General Medicine biology.organism_classification Pseudoalteromonas chemistry Biochemistry Rhodophyta Carbohydrate-binding module Carrier Proteins Biotechnology |
| Zdroj: | Journal of Biotechnology. 192:108-113 |
| ISSN: | 0168-1656 |
| Popis: | Carrageenan is a generic name for a family of polysaccharides obtained from certain species of red algae. New methods to produce useful cost-efficiently materials from red algae are needed to convert enzymatic processes into fermentable sugars. In this study, we constructed chimeric genes cCgkA and cCglA containing the catalytic domain of κ-carrageenase CgkA and λ-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain. Recombinant strains expressing the chimeric carrageenase resulted in a halo formation on the carrageenan plate by alcian blue staining. The recombinant cCgkA and cCglA were assembled with scaffoldin miniCbpA via cohesin and dockerin interaction. Carbohydrate binding module (CBM) in scaffoldin was used as a tag for cellulose affinity purification using cellulose as a support. The hydrolysis process was monitored by the amount of reducing sugar released from carrageenan. Interestingly, these results indicated that miniCbpA, cCgkA and cCglA assembled into a complex and that the dockerin-fused enzymes on the scaffoldin had synergistic activity in the degradation of carrageenan. The observed enhancement of activity by carrageenolytic complex was 3.1-fold-higher compared with the corresponding enzymes alone. Thus, the assemblies of advancement of active enzyme complexes will facilitate the commercial production of useful products from red algae biomass which represents inexpensive and sustainable feed-stocks. |
| Databáze: | OpenAIRE |
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