Determination of 4-hydroxy-2-nonenal in serum by high-performance liquid chromatography with fluorescence detection after pre-column derivatization using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2, 1,3-benzoxadiazole
| Autor: | Yuri Kurose, Naotaka Kuroda, Marwa F.B. Ali, Akina Shiokawa, Takahiro Imazato, Kaname Ohyama, Yukitaka Ueki, Eisuke Maehata, Naoya Kishikawa, Yasuha Katou |
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| Rok vydání: | 2013 |
| Předmět: |
Clinical Biochemistry
Biochemistry High-performance liquid chromatography 2-Nonenal Aldehyde Analytical Chemistry Arthritis Rheumatoid Lipid peroxidation chemistry.chemical_compound Drug Discovery Humans Molecular Biology Chromatography High Pressure Liquid Pharmacology Detection limit chemistry.chemical_classification Aldehydes Oxadiazoles Sulfonamides Chromatography Chemistry Extraction (chemistry) General Medicine Fluorescence Fluorescent labelling Spectrometry Fluorescence |
| Zdroj: | Biomedical chromatography : BMC. 28(6) |
| ISSN: | 1099-0801 |
| Popis: | 4-Hydroxy-2-nonenal (4HNE) is a major aldehyde generated during lipid peroxidation. The clinical monitoring of 4HNE in biological fluids should be useful for the early diagnosis of several diseases involving lipid peroxidation, such as rheumatoid arthritis, Parkinson's disease and cancer. In this study, an HPLC with fluorescence detection method was developed for the determination of 4HNE in human serum. The proposed method involves the extraction of 4HNE from human serum by sub-zero temperature extraction and fluorescent labeling of 4HNE with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2, 1,3-benzoxadiazole. The lower detection limit (signal-to-noise ratio = 3) of the method was 0.06 μm in serum. The proposed method was successfully applied to the measurement of 4HNE in sera obtained from patients with rheumatoid arthritis. Copyright © 2014 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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