Characteristics of mass spectrometric analyses coupled to gas chromatography and liquid chromatography for 22-oxacalcitriol, a vitamin D3 analog, and related compounds
| Autor: | Kenji Kumaki, Yoshihiko Ishitani, Masaki Ishigai |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Vitamin
Male Chromatography Molecular mass Molecular Structure Atmospheric-pressure chemical ionization General Chemistry Mass spectrometry Gas Chromatography-Mass Spectrometry Mass Spectrometry Rats Molecular Weight chemistry.chemical_compound chemistry Calcitriol Mass spectrum Animals Gas chromatography Derivatization Electron ionization Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 704(1-2) |
| ISSN: | 1387-2273 |
| Popis: | The characteristics of the mass spectra of vitamin D3 related compounds were investigated by GC-MS and LC-MS using 22-oxacalcitriol (OCT), an analog of 1,25-dihydroxyvitamin D3, and related compounds. Fragmentation during GC-MS (electron impact ionization) of TMS-derivatives of OCT and the postulated metabolites gave useful structural information concerning the vitamin D3-skeleton and its side-chain, especially with respect to the oxidation positions of metabolites. In contrast, few fragment ions were observed in LC-MS (atmospheric pressure chemical ionization), showing that LC-MS gave poor structural information, except for molecular mass. However, when comparing the signal-to-noise ratio (S/N) observed during GC-MS and LC-MS analysis for OCT in plasma extracts, the S/N in LC-MS was over ten-times greater than in GC-MS, possibly due to the low recovery on derivatization and thermal-isomerization in GC-MS. Furthermore, both the GC-MS and the LC-MS allowed the analysis of many postulated metabolites in a single injection without any prior isolation of target metabolites from biological fluids by LC. These results suggest that GC-MS and LC-MS analysis for vitamin D3 related compounds such as OCT each have unique and distinct advantages. Therefore, the complementary use of both techniques enables the rapid and detailed characterization of vitamin D3 related compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect