Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing
| Autor: | Yanni Pei, Zhennan Ma, Li Lingyu, Liyan Cui, Shen Shaofei, Defu Wang, Yanbing Niu, Long Dandan |
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| Rok vydání: | 2020 |
| Předmět: |
alcea rosea (l.)
0106 biological sciences Genetics Whole genome sequencing biology Potyvirus virus-derived small interfering rna lcsh:Plant culture biology.organism_classification 01 natural sciences Genome Virus small rna sequencing 010602 entomology Malva Sense strand lcsh:SB1-1110 Malva vein clearing virus Agronomy and Crop Science malva vein clearing virus Research Article 010606 plant biology & botany Alcea rosea |
| Zdroj: | The Plant Pathology Journal, Vol 36, Iss 5, Pp 468-475 (2020) The Plant Pathology Journal |
| ISSN: | 2093-9280 1598-2254 |
| Popis: | Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCV-SX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCV-SX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease. |
| Databáze: | OpenAIRE |
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