Production of specific IgY Helicobacter pylori recombinant OipA protein and assessment of its inhibitory effects towards attachment of H. pylori to AGS cell line
| Autor: | Ali Reza Khabiri, Katayoun Borhani, Mehrdad Behmanesh, Nima Khoramabadi, Ashraf Mohabati Mobarez |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Co-culture techniques
Microbiology law.invention Western blot law IgY Immunology and Allergy Medicine Polyacrylamide gel electrophoresis AGS cell line Pharmacology biology medicine.diagnostic_test Helicobacter pylori OipA business.industry Public Health Environmental and Occupational Health biology.organism_classification Infectious Diseases Immunization Cell culture biology.protein Recombinant DNA Immunoglobulin Y Original Article Antibody business |
| Zdroj: | Clinical and Experimental Vaccine Research |
| ISSN: | 2287-366X 2287-3651 |
| Popis: | Purpose The common triple therapy for Helicobacter pylori is challenged by the increasing cases of antibiotic resistant infections, raising the need to explore alternative therapies. Oral administration of egg yolk immunoglobulin Y (IgY) has been previously reported as a means of passive immunization therapy for H. pylori infections. In this work, we investigated the inhibitory effect of IgY on the attachment of H. pylori to AGS cell line. Materials and methods Recombinant OipA was prepared. Hens were immunized with recombinant protein three times. IgY was purified from egg yolks of immunized hens using polyethylene glycol precipitation method. The inhibitory effect of the specific immunoglobulin was evaluated in AGS cell line infected with H. pylori. Results The presence of recombinant OipA (30 kD) was confirmed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunization of hens was confirmed using enzyme-linked immunosorbent assay. The purified IgY from egg yolks were assessed using SDS-PAGE and confirmed by western blot. Conclusion The results showed that IgY-OipA had inhibitory effect on attachment of H. pylori to AGS cell line and may be utilized as a therapeutic or prophylaxis material. |
| Databáze: | OpenAIRE |
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