Cells over-expressing EAAT2 protect motoneurons from excitotoxic death in vitro
| Autor: | Peter R. Bär, Liselijn A. B. Wisman, Pierre N. E. De Graan, Freek L. Van Muiswinkel, Elly M. Hol |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pyrrolidines
Blotting Western Excitotoxicity Glutamic Acid Cell Communication Biology medicine.disease_cause Rats Sprague-Dawley medicine Paralysis Animals Humans Dicarboxylic Acids Neurotransmitter Uptake Inhibitors Amyotrophic lateral sclerosis Motor Neurons Cell Death musculoskeletal neural and ocular physiology General Neuroscience fungi Glutamate receptor Motor neuron musculoskeletal system Spinal cord medicine.disease Spinal column Coculture Techniques In vitro Rats Cell biology medicine.anatomical_structure nervous system Astrocytes medicine.symptom Genetic Engineering tissues Neuroscience |
| Zdroj: | NeuroReport. 14:1967-1970 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-200310270-00017 |
| Popis: | Amyotrophic lateral sclerosis is an incurable disease in which cerebral and spinal motoneurons degenerate, causing paralysis and death within 2-5 years. One of the pathogenic factors of motoneuron death is a chronic excess of glutamate, which exceeds its removal by astrocytes, i.e. excitotoxicity. Extra glutamate uptake in the spinal cord may slow down or prevent motoneuron death. We have engineered cells over-expressing the main glutamate transporter and tested their potential to rescue motoneurons exposed to high levels of glutamate in vitro. The engineered cells protected motoneurons in a motoneuron-astrocyte co-culture at glutamate concentrations when astrocytes were no longer capable of removing glutamate. This suggests that engineered cells, introduced into the spinal column, can help remove glutamate, thereby preventing motoneuron death. |
| Databáze: | OpenAIRE |
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