A new compartment at stereocilia tips defined by spatial and temporal patterns of myosin IIIa expression
| Autor: | Bechara Kachar, F L Erickson, Beth Burnside, Andréa C. Dosé, Mark E. Schneider, Weise Chang, Felipe T. Salles |
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| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Guinea Pigs macromolecular substances Myosin IIIA Green fluorescent protein Motor protein Mice Myosin Chlorocebus aethiops otorhinolaryngologic diseases Animals Humans Myosin Type III Cilia Actin Cells Cultured Myosin Heavy Chains Chemistry General Neuroscience Compartment (ship) Stereocilia Gene Expression Regulation Developmental Articles Cell biology Rats Protein kinase domain Ear Inner COS Cells Bass sense organs Anura Chickens |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 26(40) |
| ISSN: | 1529-2401 |
| Popis: | Class III myosins are motor proteins that contain an N-terminal kinase domain and a C-terminal actin-binding domain. We show that myosin IIIa, which has been implicated in nonsyndromic progressive hearing loss, is localized at stereocilia tips. Myosin IIIa progressively accumulates during stereocilia maturation in a thimble-like pattern around the stereocilia tip, distinct from the cap-like localization of myosin XVa and the shaft localization of myosin Ic. Overexpression of deletion mutants for functional domains of green fluorescent protein (GFP)–myosin IIIa shows that the motor domain, but not the actin-binding tail domain, is required for stereocilia tip localization. Deletion of the kinase domain produces stereocilia elongation and bulging of the stereocilia tips. The thimble-like localization and the influence myosin IIIa has on stereocilia shape reveal a previously unrecognized molecular compartment at the distal end of stereocilia, the site of actin polymerization as well as operation of the mechanoelectrical transduction apparatus. |
| Databáze: | OpenAIRE |
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